Re: [Histonet] blue flourescent nuclear dyes
If you want a blue emission you'll almost certainly
need near-UV excitation. The excitation wavelength
of a fluorochrome is always shorter than the wavelength
of the emitted light.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
Barbara Osborn wrote:
> We are just beginning flourescent staining for confocal microscopy in our lab, and our confocal microscope did not come with a UV laser, so we cannot detect DAPI as a nuclear stain. We've been told to use Draque 5 (sp) which emits blue in visible light instead of DAPI to counterstain nuclei. I cannot for the life of me find any info on this dye. Molecular Probes offers a blue flourescent nuclear dye called BOBO-1 iodide. Does anyone out there in histoland have any information about Draque 5, where to purchase it, or has anyone tried the BOBO-1 from Moleculare Probes? Or does anyone use an alternative nuclear dye? We plan to triple stain using nuclear dye/ Alexa Flour 488/ Alexa Flour 568 so I need something that does not overlap too much with the 488. Thanks.
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