Re: [Histonet] Dissolving fat around lymphnodes of breast tissue


We have used cold acetone (-20oC) for about 1 hour.  We did this for 
small fragments of fat tissue.  This could work as you would delipidate 
the fat and firm the tissue.  I am not sure if this is acceptable for a 
clinical sample.

On Feb 3, 2005, at 7:25 AM, Davala, Pam wrote:

> Does some one out there have a specialize solution for dissolving fat 
> from
> around the lymphnodes of breast or bowel tissue..... Our pathologists 
> seem
> to have a hard time taking the fat off of the nodes and it makes it 
> harder
> for the tech to cut due to unfixed fat... anyone's expertise would be
> appreciate... Is there a fixative out there from a company or is there 
> one
> that we can make.... thank you   Pam Davala HT(ASCP) Supervisor of 
> Histology
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