Hi Brianna,

We use the larger, rectangular 'boats' that hold a plastic slide
rack(24 slide capacity) We have never had boiling over. More retrieval
soln is used, but it saves you from repeating,and is more standardized.

Jo

>>> "Brianna Jackson"  02/09/05 12:39 PM >>>
Hello Everyone,

 

This question is for those of you using the Biocare Medical decloaking
chamber or the Dako Pascal decloaking chamber.  We're having trouble
with
the coplin jars boiling over during retrieval during the
depressurizing
portion of retrieval.  After the alarm sounds we turn the decloaker off
and
don't disturb the chamber until it has completely depressurized. 
We've
started using the taller coplin jars from Scienceware and it still
happens.
It doesn't seem to be dependant on the type of retrieval solution, the
amount of retrieval solution in the coplin jar, barometric pressure,
the
number of slides or whether or not we use the little perforated disk
(heat
shield) in the bottom of the decloaker.  Our patient specimens are
always
OK, being at the bottom of the slide, but we have to repeat cases that
boiled over because the control tissue at the top of the slide no
longer
stains by IHC.  

            If anyone has figured out a solution to this problem any
help
would be appreciated.

 

Thanks,

 

Brianna Jackson, BS, HTL(ASCP), QIHC

UniPath

Denver, CO

303-512-2220

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet