Re: [Histonet] Decloaking chamber problem
I use Tissue Tek rectangular plastic containers and slide racks. No problem
with overheating/overboiling, and I decloak in Dako Target Retrieval
Solution for 20 minutes, followed by a 25 minute cooldown inside the cooker.
U of MN Vet Diag Lab
----- Original Message -----
From: "Brianna Jackson"
Sent: Wednesday, February 09, 2005 11:39 AM
Subject: [Histonet] Decloaking chamber problem
> Hello Everyone,
> This question is for those of you using the Biocare Medical decloaking
> chamber or the Dako Pascal decloaking chamber. We're having trouble with
> the coplin jars boiling over during retrieval during the depressurizing
> portion of retrieval. After the alarm sounds we turn the decloaker off
> don't disturb the chamber until it has completely depressurized. We've
> started using the taller coplin jars from Scienceware and it still
> It doesn't seem to be dependant on the type of retrieval solution, the
> amount of retrieval solution in the coplin jar, barometric pressure, the
> number of slides or whether or not we use the little perforated disk (heat
> shield) in the bottom of the decloaker. Our patient specimens are always
> OK, being at the bottom of the slide, but we have to repeat cases that
> boiled over because the control tissue at the top of the slide no longer
> stains by IHC.
> If anyone has figured out a solution to this problem any help
> would be appreciated.
> Brianna Jackson, BS, HTL(ASCP), QIHC
> Denver, CO
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