I have seen this occasionally with various antibodies. It seems to be a 
combination of too short a fixation and HIER. Properly fixed control 
material does not show this false positivity.

Katri

Katri Tuomala
Hamilton, Ontario, Canada
----- Original Message ----- 
From: "Brandon Stokes" 
To: 
Sent: Tuesday, February 01, 2005 9:26 PM
Subject: [Histonet] CD-20 nuclear staining....


> Hello fellow histo-netters,
>
> I was wondering if anyone else has ever experienced nuclear staining 
> occuring when using the CD-20 antibody.  The tonsil control will look 
> great....then you look at the patient tissue(almost always skin for us) 
> and some of the nuclei of the keratinocytes in the epidermis and even some 
> nuclei in the dermis might light up bright red(we use AEC).  You can 
> actually see the nuclear detail very well.  This problem doesn't happen 
> all the time so I am not sure what it could be.
>
> Any advice would be greatly appreciated.
>
>
> Brandon Stokes, HT(ASCP)
> Lab Manager
> Cutting Edge Histology Services, LLC
>
>
>
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