Re: [Histonet] Alcian Blue problem

From:John Kiernan

It also stains ceramic sinks pretty well! The dye cations
are supposed to stick to the silicate anions of the glass
or pot. The slide adhesive paper is:

Archimbaud E, Islam A, Preisler HD (1986) Alcian blue 
method for attaching glycol methacrylate sections to 
glass slides. Stain Technology 61: 121-123.

To obtain a positively charged surface this way you'd 
have to stain the slides immediately before using, and
wash in something a bit acidic. Anything as basic as
water removes the pendant cationic groups from bound
alcian blue, leaving behind uncharged and completely
insoluble copper phthalocyanine. I don't have that
issue of Stain Technology to hand, so cannot check up
on the method.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
Geoff McAuliffe wrote:
>     Many years ago I noticed that Alcian Blue stained/coated the whole
> slide to a slight extent. I seem to remember a short paper in Stain
> Technology, the former name of Biotechnique and Histochemistry, that
> described Alcian Blue as a coating for slides that helped attach sections.
> Geoff
> Tom McNemar wrote:
> >We have started to see a hazy/cloudiness to our alcian blue PAS slides.  We
> >don't see this on any other slide/stain.  We use the same slides, reagents,
> >and procedure that we have always used.  The stain works fine and the haze
> >is on the entire slide, not just the tissue.  We routinely use the
> >Fisherbrand red control slides and have tried other slides and still have
> >the same problem.  I use the Poly Scientific reagents (alcian blue ph 2.5)
> >and have switched them all out for new  reagents but still have the haze.
> >We have switched out, altered, and tried everything I can think of.  I am
> >totally baffled.  Hope you folks have some ideas.
> >
> >Tom Mc Nemar HT(ASCP)
> >Histology Supervisor
> >Licking Memorial Hospital
> >Newark, Ohio 43055
> >
> >
> >_______________________________________________
> >Histonet mailing list
> >
> >
> >
> >
> >
> >
> --
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583; fax: -4029
> **********************************************
> _______________________________________________
> Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>