Re: [Histonet] Alcian Blue problem

From:Geoff McAuliffe

    Many years ago I noticed that Alcian Blue stained/coated the whole 
slide to a slight extent. I seem to remember a short paper in Stain 
Technology, the former name of Biotechnique and Histochemistry, that 
described Alcian Blue as a coating for slides that helped attach sections.


Tom McNemar wrote:

>We have started to see a hazy/cloudiness to our alcian blue PAS slides.  We
>don't see this on any other slide/stain.  We use the same slides, reagents,
>and procedure that we have always used.  The stain works fine and the haze
>is on the entire slide, not just the tissue.  We routinely use the
>Fisherbrand red control slides and have tried other slides and still have
>the same problem.  I use the Poly Scientific reagents (alcian blue ph 2.5)
>and have switched them all out for new  reagents but still have the haze.
>We have switched out, altered, and tried everything I can think of.  I am
>totally baffled.  Hope you folks have some ideas.
>Tom Mc Nemar HT(ASCP)
>Histology Supervisor
>Licking Memorial Hospital
>Newark, Ohio 43055
>Histonet mailing list

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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