RE: [Histonet] another background / non-specific staining problem


I had a similar problem when staining non-perfused sheep placenta 
with eNOS and someone (I think it was Gayle) on Histonet suggest 
putting my slides in 1% NaOH (the other 99 being ethanol) 
solution. I've been putting my slides in 1% NaOH for 15 min after 
the peroxide and it has worked well.
"You are a child of the universe, no less than the trees and the 
You have a right to be here and whether or not it is clear to you, 
no doubt the universe is unfolding as it should. Therefore be at 
peace with G-d, whatever you conceive Him to be. And whatever your 
labors and aspirations,in the noisy confusion of life, keep peace 
in your soul.-Max Ehrmann,"Desiderata"

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