Tom,

I have used a very good modification of Gabriel-Steiner's original technique
that works well.  It was in an old issue of Histo-Logic, January 1988.  It
can be found at http://www.sakura-americas.com/histologic/pdf/88_jan.pdf and
requires no developing with almost not background staining.  Spirochetes are
easily seen.  You can also do IHC which demonstrates them nicely, I have
gotten the antibody from Biocare in the past.

Shirley Powell

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Thomas
Wood
Sent: Friday, February 11, 2005 9:54 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Warthin-Starry protocol needed


Can anyone give me a good Warthin-Starry protocol for spirochetes, ours no
longer does the job.

Tom Wood BS HT
Michigan State University
Veterinary Medicine


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