RE: [Histonet] Rubber cement (for in situ)

From:"Elizabeth Chlipala"

We use Frame-Seal from MJ Research, Inc. www.mjr.com (888)735-8437

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of - -
Sent: Thursday, February 03, 2005 10:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Rubber cement (for in situ)

Hello,
 
I am starting to work up a protocol for in situ hybridization and
discovered I need to seal my slides because of the high temps and long
incubation.  Can anyone help me with the following questions:  
 
a. I noticed the rubber cement is quite fluid.  Using the brush that it
comes with the can (or another method), how does one go about applying
it after your sample is coverslipped?
 
b. I've heard about fumes ... what concerns should I have; safety
precautions should I take? (my normal range of temperature will be
between 43 and 55 degrees Celcius within a Boekel Slide Moat or all
purpose incubator)
 
c. Are there better methods to use when sealing slides? 
 
d. What is the best way to remove the rubber cement/coverslip after
performing hybridization?
 
THANK YOU ALL VERY MUCH!!!!!

		
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