RE: [Histonet] IL-6

From:"Elizabeth Chlipala"


I have stained for IL-6 on formalin fixed, formic acid decalcifed rat
joints.  I use the antibody from Abcam ab6672.  I used proteinase K
digestion with envision +.  This antibody is directed against human
IL-6.  My dilution was 1:300 for 30 minutes at RT.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
[] On Behalf Of
Margaryan, Naira
Sent: Wednesday, February 02, 2005 9:53 AM
Subject: [Histonet] IL-6

Hi everyone,


Is anybody have been tried to use IL-6 for the FFPE Breast tissue
(normal and carcinoma)? If yes, what protocol did you use?

I have a Monoclonal anti-human IL-6 Ab from R&D (cat#: MAB206) and I
tried to IHC with DAB staining on the breast tissue. Unfortunately, I
didn't get any staining.


Is anybody can send me FFPE tonsil tissue as a control?





Naira V. Margaryan, Ph.D, D.V.M.

Research Associate II

Children's Memorial Research Center

2300 Children's Plaza, Box 222

Chicago, IL 60614-3394

Tel: 773-880-4000/5-6740

Fax: 773-755-6594


For Express Mail: 

CMRC, Room C.473

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Chicago, IL  60614-4314


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