[Histonet] peroxidase/biotin block before or after permeabilization ?
I am performing peroxidase-DAB staining on cultured cells and was
wondering whether peroxidase and biotin blocking should be performed
before or after the permeabilization step with PBS + Tween ? Does
permeabilization before blocking improve blocking due to better
penetration ? My protocol is : peroxidase block with 0.3% H202 in
methanol for 30 min. at RT, followed by avidin-biotin block from Dako,
followed by PBS + Tween for 15 min. , followed by 30 min. block with
fish skin gelatin in PBS.
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