[Histonet] another background / non-specific staining problem: Thank you all


Dear all
Thank you very much for your prompt reply to my problem. I have received
several responses. Now I am trying to follow the suggestions.

Thank you all of you for your nice suggestions.


--------- Mensagem Original --------
To: GUTIERREZ, JUAN , Subratab
, histonet@lists.utsouthwestern.edu

Subject: RE: [Histonet] another background / non-specific staining problem
Date: 02/02/05 09:48

> I had a similar problem when staining non-perfused sheep placenta
> with eNOS and someone (I think it was Gayle) on Histonet suggest
> putting my slides in 1% NaOH (the other 99 being ethanol)
> solution. I've been putting my slides in 1% NaOH for 15 min after
> the peroxide and it has worked well.
> --
> "You are a child of the universe, no less than the trees and the
> stars
> You have a right to be here and whether or not it is clear to you,
> no doubt the universe is unfolding as it should. Therefore be at
> peace with G-d, whatever you conceive Him to be. And whatever your
> labors and aspirations,in the noisy confusion of life, keep peace
> in your soul.-Max Ehrmann,"Desiderata"

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