[Histonet] RE: endogenous peroxidase blues

From:"C.M. van der Loos"

   Hi all,

   On  Patty's request here is a little list of antibodies that were once
   tested  with the Dual Endogenous Enzyme Block reagent (Dako S2003) and
   the staining result.

   GFAP      (RbxHu)     (Dako)              rat  duodenum    no  problem
   OX6       (MsxRt)   (Serotec)         rat
   duodenum    negative
   OX17      (MsxRt)   (Serotec)         rat
   duodenum    weak
   OX42      (MsxRt)   (Serotec)         rat
   duodenum    negative          &nbs! p;

   These  antibodies  were not selected or something. Just, at some point
   this  blocking  kit  was  interesting to evaluate. As you see staining
   with  6/9  antibodies  resulted into  staining problems. Don't get too
   scared of this basically good product, but you guys are warned now for
   eventual failures!

   Chris van der Loos, PhD
   Dept. of Pathology
   Academical Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands

   phone:  +31 20 5665631
   fax:    +31 20 6960389
   e-mail: [1]c.m.vanderloos@amc.uva.nl

   -----Original Message-----
   Chris  can you share with us which antigens didn't survive this enzyme
   in your hands.
   -----Original Message-----
   From: histonet-bounces@lists.utsouthwestern.edu
   [[2]mailto:histonet-bounces@lists.utsouthwestern.edu]   On  Behalf  Of
   C.M. van der
   Sent: Monday, January 31, 2005 12:50 AM
   To: histonet@lists.utsouthwestern.edu
   Subject: [Histonet] RE: endogenous peroxidase blues
      Hi all,
      Before everybody is trying to solve their problem of endogenous
      peroxidase activity with Dual Enzyme Blocking Reagent as Veronique
      wrote us, I would like to make a comment. This stuff works great
      indeed in many cases, that's true. But I also found some! antigens


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