[Histonet] Immunofluorescence staining artefact?
I'm performing a double fluorescent staining (Cy3 + FITC) of adherent cells
fixed with 2%paraformaldehyde. In my stained cells, apart of the typical
staining pattern i see small, very bright Cy3 dots, moving fast with a kind
of Brownian motion. Their size is in the microscope resolution area (viewed
with 100x magn.) Nothing can be spotted with the phase contract, so it's not
bacteria. I tried filtering, centrifuging, nothing... It seems, that a long
washing reduces this artefact. Are these Cy3 clumps?
Anyone knows what it is and how to get rid of it?
Any help appreciated,
P. M. Krawczyk
Dept. of Cell Biology and Histology
Academic Medical Centre
University of Amsterdam
Meibergdreef 15, room M3-107
1105 AZ, Amsterdam
Tel: +31 20 5668746
Fax: +31 20 6974156
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