[Histonet] CLC1 Ab labeling

From:Danielle Jin

Hello! Histonetters,

I have been trying to use every commercially availabled chloride channel
antibodys (CLC1) to label frosen human muscle sections without success.
Would anyone give me any suggesions what to do/try?
I tried different dilutions of the CLC1, include straight, with vector
ABC kit and plus TSA amplification kit from PerkinElmer.  And I tried
use Cy3-goat anti-rabbit IgG as secondary Ab.  None worked, the signals
are very fade with high backgrounds.
Any suggestions will be appreciated.

Danielle Jin

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