Re: [Histonet] ki67 or pcna (anti-mouse)

From:"Andi Kappeler"

Hi Daniel

I assume you want to stain (para)formaldehyde-fixed, paraffin-embedded mouse
tissue, right? Try rat-anti-mouse Ki-67, clone TEC-3 (DakoCytomation M
7249). We use it after HIER in a pressure cooker with citrate buffer, pH
6.0. Working dilution is 1:100 - 1:200, when using a 3-step protocol like
ABC, LSAB or similar. Visualization: use a x-a-rat Ig/Biotin secondary that
has been absorbed with mouse Ig to avoid demonstration of endogenous mouse
Ig in your tissue sections (e.g. DakoCytomation E 0468 at 1:300 - no, I
don't have any shares).
Good luck
Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

----- Original Message ----- 
From: "DANIEL EBERHARD" 
To: 
Sent: Monday, February 23, 2004 11:22 AM
Subject: [Histonet] ki67 or pcna (anti-mouse)


> Dear All,
> sorry if this question has been ask before,
> Im searching for a ki67 or pcna (anti-mouse) antibody
> which works on pFA fixed tissue.
> Thanks for any hints.
> Daniel.
>
> -- 
>                              (-)-(-)
> ---------------------------  \"/ ---
> Dr. Daniel Eberhard          =V=
>
> Developmental Biology
> & Molecular Pathology
>
> Graduate Programe on Pattern Formation
>
> University of Bielefeld
> D 33501 Bielefeld/Germany
>
> FAX: xx49(0)521-106-5654   (-)-(-)
> ---------------------------  \"/ ---
>                              =V=
>
>
>
>
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