After trying several GFP ABīs, I use MBLīs rabbit polyclonal GFP=20
-antibody (No 598).
It works perfect on pFA fixed tissue in 1:100 to 1:500 dilutions without 
any special section
treatments.
Daniel

>I use Molecular Probe's rabbit poly A6455; superb on FFPW sections after
>TRIS pH10 M/W treatment. Around 1/700 for ABC- DAB and indirect fluorescence
>using  ALEXA fluorochromes( pwax sections mounted in Mowiol containing DABCO
>and Azide to retard fading)
>
>
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>


-- 
                             (-)-(-)
---------------------------  \"/ ---
Dr. Daniel Eberhard          =V=

Developmental Biology 
& Molecular Pathology 

Graduate Programe on Pattern Formation

University of Bielefeld
D 33501 Bielefeld/Germany

FAX: xx49(0)521-106-5654   (-)-(-)
---------------------------  \"/ ---
                             =V=   




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