The next issue of HistoLogic will include a comprehensive article by Gayle Callis on the handling of tissues intended for cryosectioning. Gayle may respond to this query herself but have you considered perfusing the lung with OCT before freezing? The OCT can, in this manner, occupy the spaces filled with air that will provide the support the tissue needs during sectioning.



Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, SC 29425
Ph: 843-792-6353
fax: 843-792-8974

>>> "Ing. Barbara Bublava"  02/23/04 08:23AM >>>
Hi everybody,

I m having problems with the cryosectioning of lung, the air in it causes
some trouble. I had the idea to get rid of it using vaccum but our
pathologist thinks that this woul affekt oedemas.

I have to section formalin-fixated and native lung. I did try to work
at -20°C for native and -30°C for fixated lung.

I would be very glad for any hints.
Thanx in advance

Barbara Bublava

Inst. f. Forensic Medicine
Vienna



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