RE: Re: Fwd: [Histonet] Isopentane

From:Tony Henwood


We do not have this problem. 
When we freeze specimens in liquid nitrogen we shake the specimen and chuck
vigorously in the liquid nitrogen and no ice crystal artifact.

My experience is that Liquid nitrogen is faster than isopentane/N2.

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: Barry R Rittman []
Sent: Wednesday, 25 February 2004 4:41 AM
Subject: RE: Re: Fwd: [Histonet] Isopentane

The purpose of using isopentane cooled with liquid nitrogen is to
dissipate heat quickly and thus prevent the formation of a vapor barrier
around the tissues. When nitrogen is used alone, the vapor barrier that
is formed significantly decreases the rate of cooling and therefore
increases the possibility of ice crystal formation and tissue damage.

-----Original Message-----
[] On Behalf Of Atoska
S. Gentry
Sent: Tuesday, February 24, 2004 11:24 AM
To: Histonet
Subject: Fwd: Re: Fwd: [Histonet] Isopentane

Hello, I'm not aware of a procedure for quick freezing in isopentane
we quick freeze by immerging the sample in isopentane chilled by liquid 
nitrogen. Please will you share with me your protocol for quick freezing
isopentane. Thanks, Atoska

>X-Mailer: Novell GroupWise Internet Agent 6.5.1
>Date:  Tue, 24 Feb 2004 10:50:58 -0600
>From: "Nita Searcy" 
>Subject: Re: Fwd: [Histonet] Isopentane
>Isopentane is used to "quick freeze" frozen sections- can't liquid 
>nitrogen be use in its place? No specialty work (muscles, etc.) but 
>routine frozens.
> >>> "Atoska S. Gentry"  02/24/04 10:12AM
> >>>
>Hello, I don't believe isopentane can be substituted for liquid 
>nitrogen, they're usually used in conjunction. However, dry ice can be
>for liquid nitrogen. Best wishes. Atoska
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> >For Vinny- or anyone. I am trying to substitute liquid nitrogen for 
> >Isopentane in the frozen lab due to volatility. Could not access 
> >Histologic article from May regarding laboratory accident. Anyone 
> >have any other incidents?  Help would be appreciated.
> >
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>Atoska S. Gentry B.S., HT(ASCP)
>Research Assistant III
>Scott-Ritchey Research Center
>College of Veterinary Medicine
>Auburn University, AL  36849
>Phone# (334)844-5579  Fax# (334)844-5850
>Histonet mailing list

Atoska S. Gentry B.S., HT(ASCP)
Research Assistant III
Scott-Ritchey Research Center
College of Veterinary Medicine
Auburn University, AL  36849
Phone# (334)844-5579  Fax# (334)844-5850 

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