RE: [Histonet] ki67 or pcna (anti-mouse)

From:"Elizabeth Chlipala"

Daniel

Dako has a mouse anti Ki-67 (clone TEC-3) that works well in formalin
fixed paraffin embedded tissue.   Steam retrieval, primary antibody 1:50
for 30 minutes room temp, then Dako's Biotinylated Rabbit anti-rat at
1:200 for 30 minutes,  then streptavidin/HRP for 30  minutes, if you
find you need more amplification you can substitute the streptavidin/HRP
with Dako's Envision + rabbit, but you might need to change the dilution
of the primary.  Hope this helps.  

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592 
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
lizchlipala@premierhistology.com
www.premierhistology.com
 
Ship to Address:
Premier Histology Laboratory
University of Colorado
MCBD, Room A3B40
Boulder, Colorado 80309
 
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-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DANIEL
EBERHARD
Sent: Monday, February 23, 2004 3:23 AM
To: histonet@pathology.swmed.edu
Subject: [Histonet] ki67 or pcna (anti-mouse)

Dear All,
sorry if this question has been ask before,
Im searching for a ki67 or pcna (anti-mouse) antibody
which works on pFA fixed tissue.
Thanks for any hints.
Daniel.

-- 
                             (-)-(-)
---------------------------  \"/ ---
Dr. Daniel Eberhard          =V=

Developmental Biology 
& Molecular Pathology 

Graduate Programe on Pattern Formation

University of Bielefeld
D 33501 Bielefeld/Germany

FAX: xx49(0)521-106-5654   (-)-(-)
---------------------------  \"/ ---
                             =V=   




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