RE: [Histonet] fat fixation

From:"Elizabeth Chlipala"


We used a technique that has been published in the Journal of
Histotechnology 1999, volume 22 - by Dr Ruby.  We would use this on
lumpectomy and breast biopsy specimens.  We would ink the entire
specimens with marking ink and then slice thin sections through the
entire breast biopsy placing each section on a folded paper towel in the
order that it was sectioned.  We would then roll up the paper towel with
the breast slices within it, secure it with rubber bands and place it in
alcoholic formalin overnight, we would then continue the next morning
with grossing in of  the specimen.  The sections cut beautifully.  I
hope this helps.

Elizabeth A. Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592 
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
Premier Histology Laboratory
University of Colorado
MCBD, Room A3B40
Boulder, Colorado 80309
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-----Original Message-----
[] On Behalf Of Joyce
Sent: Wednesday, February 25, 2004 12:47 PM
To: Histonet
Subject: [Histonet] fat fixation

We are fixing our breast cases overnight in 10% buffered formalin, then
placing the cassettes into Penfix, then process normally that night.
 I have a penfix station on the processor after two formalins. We are
still having trouble with the breast tissue that is extremely fatty.
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