RE: [Histonet] endogenous peroxidases in liver sections
From: | "Edmondson David (RBV) NHS Christie Tr" |
Following on from Patsy,
we went on to using DAKO Envision detection system kit because of all the
Biotin blocking that we were doing. And now things are clean.
3% Hydrogen Peroxide was a standard system for blocking endogenous
peroxidase. Commercially available products are in the catalogues
And I remember Methanolic peroxide years ago, where we had 4ml of 30%
Hydrogen peroxide in 250ml of Methanol and blocked for thirty minutes!
Which seems a little aggressive/desparate.
Dave
Manchester
-----Original Message-----
From: Cathy Malcontenti-Wilson [mailto:cmalc@unimelb.edu.au]
Sent: 25 February 2004 22:36
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] endogenous peroxidases in liver sections
Hi,
Has anyone got any good method for blocking endogenous peroxidases in mouse
liver sections? We seem to have a lot of red and white blood cells and
plasma coming up in our negatives. We are using a Zymed MAX kit and SP kit
(mouse on mouse and universal kits). Some pearls of wisdom will be very
much appreciated.
Thanks
Cat
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