RE: [Histonet] endogenous peroxidases in liver sections

From:"Edmondson David (RBV) NHS Christie Tr"

Following on from Patsy,
we went on to using DAKO Envision detection system kit because of all the
Biotin blocking that we were doing.  And now things are clean.
3% Hydrogen Peroxide was a standard system for blocking endogenous
peroxidase.  Commercially available products are in the catalogues
And I remember Methanolic peroxide years ago, where we had 4ml of 30%
Hydrogen peroxide in 250ml of Methanol and blocked for thirty minutes!
Which seems a little aggressive/desparate.   

-----Original Message-----
From: Cathy Malcontenti-Wilson []
Sent: 25 February 2004 22:36
Subject: [Histonet] endogenous peroxidases in liver sections

Has anyone got any good method for blocking endogenous peroxidases in mouse 
liver sections? We seem to have a lot of red and white blood cells and 
plasma coming up in our negatives. We are using a Zymed MAX kit and SP kit 
(mouse on mouse and universal kits). Some pearls of wisdom will be very 
much appreciated.

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