RE: [Histonet] angiogenesis study

From:"Mikael Niku"

Dear Wendy,

to recommend a tissue to look at, I think you need to be a bit more
Are you expecting a systemic effect on angiogenesis? Or are you going to
apply the drug locally and look for local effects? Are you looking for
increased angiogenesis, or inhibition? In the latter case, you of course
need a tissue where you either have angiogenesis normally (which are not
too many in a healthy animal!), or where you can easily induce it.

I think mouse or rabbit ear is a common model to study angiogenesis
induction. The staining methods recommended by Nick are probably the
most commonly used.

+  Mikael Niku		 
+  University of Helsinki, Dept. Basic Veterinary Sciences
+  URL:

- Mitäkö mieltä olen länsimaisesta sivistyksestä?
  Minusta se olisi erinomainen ajatus!

> -----Original Message-----
> From: 
> [] On Behalf 
> Of Wendy England
> Sent: 20. helmikuuta 2004 0:43
> To:
> Subject: [Histonet] angiogenesis study
> Dear histonetters,
> We are going to have  a  study that check angiogenesis after 
> drug treatment. 
> I have three questions: a, what tissue we should look at, is 
> eye a good one? 
> b, how to fix this tissue? c, what stain, or what antibody, 
> we should use?
> Any advice is highly appreciated.
> Wendy
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