RE: [Histonet] alkaline phosphatase

From:Tony Henwood

Try using cobalt chloride to change the colour of the DAB

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: Martin Weber []
Sent: Thursday, 19 February 2004 6:20 AM
Subject: [Histonet] alkaline phosphatase

I might need to do a dual staing procedure on paraformaldehyde fixed 
fresh frozen sections. One marker will be stained with peroxidase (DAB). 
As a second marker for IHC I read about alkaline phosphatase (FastRed) 
which gives rise to a red color in addition to the brown color of the 
peroxidase staining. To me this combination seems to be a little bit 
unfortunate because the colors are sort of overlapping.
Does anybody know of an alternative and complimentary method for dual 
staining which might give rise to a different color other than red ? 
Unfortunately due to special circumstances Immunofluorecence is not an 
Any help is much appreciated. Thanks a lot !

Martin Weber

The Scripps Research Institute
Dept. of Molecular and Experimental Medicine
10550 North Torey Pines Road
La Jolla, 92037
Phone: 858-784-8612
Fax: 858-784-2030

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