RE: [Histonet] alkaline phosphatase

From:"Connolly, Brett M"

Another possibility comes to mind. You might consider changing the
peroxidase substrate. I have used TrueBlue Peroxidase Substrate from
Kirkegaard & Perry Laboratories, it's more sensitive than DAB and if you
play with the conditions you can end up with a turquoise reaction product.


Brett M. Connolly, Ph.D.
Merck & Co., Inc.
MRL, Imaging Research
PO Box 4
West Point, PA 19486
PH 215-652-2501
fax. 215-652-2075

-----Original Message-----
[] On Behalf Of Martin Weber
Sent: Wednesday, February 18, 2004 2:20 PM
Subject: [Histonet] alkaline phosphatase

I might need to do a dual staing procedure on paraformaldehyde fixed 
fresh frozen sections. One marker will be stained with peroxidase (DAB). 
As a second marker for IHC I read about alkaline phosphatase (FastRed) 
which gives rise to a red color in addition to the brown color of the 
peroxidase staining. To me this combination seems to be a little bit 
unfortunate because the colors are sort of overlapping.
Does anybody know of an alternative and complimentary method for dual 
staining which might give rise to a different color other than red ? 
Unfortunately due to special circumstances Immunofluorecence is not an 
Any help is much appreciated. Thanks a lot !

Martin Weber

The Scripps Research Institute
Dept. of Molecular and Experimental Medicine
10550 North Torey Pines Road
La Jolla, 92037
Phone: 858-784-8612
Fax: 858-784-2030

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