[Histonet] alkaline phosphatase
I might need to do a dual staing procedure on paraformaldehyde fixed
fresh frozen sections. One marker will be stained with peroxidase (DAB).
As a second marker for IHC I read about alkaline phosphatase (FastRed)
which gives rise to a red color in addition to the brown color of the
peroxidase staining. To me this combination seems to be a little bit
unfortunate because the colors are sort of overlapping.
Does anybody know of an alternative and complimentary method for dual
staining which might give rise to a different color other than red ?
Unfortunately due to special circumstances Immunofluorecence is not an
Any help is much appreciated. Thanks a lot !
The Scripps Research Institute
Dept. of Molecular and Experimental Medicine
10550 North Torey Pines Road
La Jolla, 92037
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