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Hello Laurie,

It's been a while since I've done the fixation but I would cast my vote
for 4% paraformaldehyde (fresh) in PBS buffer. Perfusion would be ideal,
but if the tissue is small enough, immersion works fine. We usually
immersed/fixed the tissue in cool fixative (i.e. refrigerated
temperatures) until it was time to process and paraffin infiltrate in an
automated processor. At that point, routine automated processing in 10%
NBF was the norm (if any buffered formalin steps were used at all).

Hope this helps,
Todd


Todd Sherman
President
HistoSoft Corporation
www.histosoft.com   - Home
www.myhistosoft.com - Member Services

histonet-request@lists.utsouthwestern.edu wrote:

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|   10. Best fixative for Mouse lung (Starkus, Laurie)
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Message: 10
Date: Thu, 19 Feb 2004 12:43:31 -0500
From: "Starkus, Laurie" 
Subject: [Histonet] Best fixative for Mouse lung
To: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain;	charset="iso-8859-1"

For those of you doing rodent lung, what is your preferred fixative and I
assume perfusion is your best method?
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