[Histonet] RE: Histonet Digest, Vol 3, Issue 30 [Scanned By SOPHOS Anti-Viru s]
| From: | G.A.McHardy@arh.grampian.scot.nhs.uk |
We are trying to source a meter suitable for checking air levels of Xylene.
Does anyone know of a UK supplier?
Thanks
Graham McHardy Path Dept ARI Aberdeen
G.A.McHardy@arh.Grampian.scot.nhs.uk
> -----Original Message-----
> From: histonet-request@lists.utsouthwestern.edu
> [SMTP:histonet-request@lists.utsouthwestern.edu]
> Sent: 24 February 2004 18:00
> To: histonet@lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 3, Issue 30 [Scanned By SOPHOS
> Anti-Virus]
>
> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
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>
> Today's Topics:
>
> 1. Would Recruiter who had opening in Indianapolis, IN
> (Dunn-Jena, Patsy A)
> 2. Available Leitz 1400 Sledge Microtome (* B.I.C*)
> 3. Using Poly-L-Lysine to coat slides (Coulter, Diane)
> 4. RE: ki67 or pcna (anti-mouse) (C.M. van der Loos)
> 5. Yale University - Opening (Ramona Tolliver)
> 6. TRAP stain components (Wester, Martha)
> 7. Perforin (Elaine Dooley)
> 8. Perforin (Elaine Dooley)
> 9. Yale University - Opening (Ramona Tolliver)
> 10. CMC mounting media (marilyn.johnson@gov.ab.ca)
> 11. unsubscribe please (Jeremy Browne)
> 12. (no subject) (Linresearch@aol.com)
> 13. CD21 (Linresearch@aol.com)
> 14. cutting frozen sections of myocardium (Shaumik Adhya)
> 15. filtering stains (what paper?)
> (Nancy.Walker@sanofi-synthelabo.com)
> 16. substance p (Mike Bromley)
> 17. Isopentane (Nita Searcy)
> 18. filtering stains (what paper?)
> (Nancy.Walker@sanofi-synthelabo.com)
> 19. (no subject) (Myri37@aol.com)
> 20. RE: filtering stains (what paper?) (Gary Gill)
> 21. RE: filtering stains (what paper?) (Gary Gill)
> 22. Fwd: [Histonet] cutting frozen sections of myocardium
> (Atoska S. Gentry)
> 23. Re: substance p (John C. Dennis)
> 24. Fwd: [Histonet] Isopentane (Atoska S. Gentry)
> 25. please unsubscribe (Martha Ward)
> 26. Fwd: Re: Fwd: [Histonet] Isopentane (Atoska S. Gentry)
> 27. RE: Re: Fwd: [Histonet] Isopentane (Barry R Rittman)
> 28. Re: substance p (Dana Settembre)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 23 Feb 2004 13:07:14 -0500
> From: "Dunn-Jena, Patsy A"
> Subject: [Histonet] Would Recruiter who had opening in Indianapolis,
> IN
> To: "histol-histopathol@lg.ehu.es"
> Message-ID:
>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> Please contact me privately at padunnje@hotmail.com. I would appreciate
> it.
>
> Patsy Dunn-Jena
> padunnje@hotmail.com
>
>
>
> ------------------------------
>
> Message: 2
> Date: Mon, 23 Feb 2004 18:19:11 +0000
> From: "* B.I.C*"
> Subject: [Histonet] Available Leitz 1400 Sledge Microtome
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; format=flowed
>
> Leitz 1400 Sledge Microtome in excellent condition.
>
> Looking for best offer.
>
> Mike MacDougall
> (800)783-9424 Ext.114
>
> _________________________________________________________________
> Dream of owning a home? Find out how in the First-time Home Buying Guide.
> http://special.msn.com/home/firsthome.armx
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Mon, 23 Feb 2004 13:23:11 -0500
> From: "Coulter, Diane"
> Subject: [Histonet] Using Poly-L-Lysine to coat slides
> To: "'histonet@lists.utsouthwestern.edu'"
>
> Message-ID:
>
>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> We're presently a commercially prepared, liquid Poly-L-Lysine as an
> adhesive
> for some of our slides. Is anyone using a dry form to make up their own
> solution? If so, where are you purchasing from and what concentration do
> you use? (We use these slides for special stains.)
>
>
> Thanks in advance,
> Diane
>
> Diane Coulter, RIH Histology
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Mon, 23 Feb 2004 19:37:26 +0100
> From: "C.M. van der Loos"
> Subject: [Histonet] RE: ki67 or pcna (anti-mouse)
> To: histonet@pathology.swmed.edu
> Message-ID: <3958ab398a63.398a633958ab@amc.uva.nl>
> Content-Type: text/plain; charset=iso-8859-1
>
> Daniel,
> During the Animal Research Kit workshop at NSH 2003 in Louisville last
> October I used a wonderful Ki67 antibody named CDC47 from Neomarkers. It
> worked wonderful on FFPE mouse intestinal tissue sections after EDTA9.0
> HIER with all workshop attendees! Because CDC47 is raised in mouse, you
> need the ARKit for detection.
> Hope this helps.
> Chris vand er Loos, PhD
> Dept. of Pathology
> Academical Medical Center
> Amsterdam - The Netherlands
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DANIEL
> EBERHARD
> Sent: Monday, February 23, 2004 3:23 AM
> To: histonet@pathology.swmed.edu
> Subject: [Histonet] ki67 or pcna (anti-mouse)
>
> Dear All,
> sorry if this question has been ask before,
> I´m searching for a ki67 or pcna (anti-mouse) antibody
> which works on pFA fixed tissue.
> Thanks for any hints.
> Daniel.
> (-)-(-)
> --------------------------- \"/ ---
> Dr. Daniel Eberhard =V=
>
> Developmental Biology
> & Molecular Pathology
>
> Graduate Programe on Pattern Formation
>
> University of Bielefeld
> D 33501 Bielefeld/Germany
>
> FAX: xx49(0)521-106-5654
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 23 Feb 2004 13:47:49 -0500
> From: Ramona Tolliver
> Subject: [Histonet] Yale University - Opening
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <5.2.0.9.2.20040223134732.0122d248@email.med.yale.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
> Good afternoon,
>
> Yale University has a 2nd Shift Histology Supervisor Position available=20
> from 3:00 -11:00 p.m. If you know of anyone who may be interested in a=20
> great opportunity with advancement potential, please forward this
> information on to them.
>
> The posting is located at :
> http://websrv.its.yale.edu/hr/cgi-bin/printjob.plx?file=mp+2&job=124471.
>
> Salary starts at 65K and is commensurate with experience. Relocation
> assistance is available.
>
> Thank you for your time!
>
> Ramona Tolliver
>
>
> Ramona E. Tolliver
> Human Resource Manager
> Yale University School of Medicine
> Department of Pathology
> 310 Cedar Street
> New Haven, CT 06520-8023
> Telephone: (203) 785-6689
> Fax: (203) 785-7303
>
> ------------------------------
>
> Message: 6
> Date: Mon, 23 Feb 2004 16:19:47 -0500
> From: "Wester, Martha"
> Subject: [Histonet] TRAP stain components
> To:
> Message-ID:
> <83899F0EC7671543B305FB5694024DE6BAD2BE@medimmune4.medimmune.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I am gearing up to do some TRAP staining, over a period of time. Can
> anyone tell me if it is possible to make up and store the various stock
> solutions, how to store them and for what period of time they remain
> usable? It would help me out a great deal not to have to devote 1-2 hrs
> just before every incubation period to make up fresh stocks. Thanks for
> your help!
>
> Martha S. Wester
> Associate Scientist
> MedImmune, Inc.
> Gaithersburg, MD 20878
> (240) 632-4794
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Mon, 23 Feb 2004 16:43:32 -0500
> From: "Elaine Dooley"
> Subject: [Histonet] Perforin
> To:
> Message-ID:
> Content-Type: text/plain; charset=US-ASCII
>
> Dear histonetters,
>
> Does anyone out there have a antibody called Perforin that works well in
> formalin fixed paraffin embedded tissues?
>
>
> Elaine Dooley
> Shands Teaching Hospital
> Gainesville FL
>
> 352-265-0111 ext 72117
>
>
>
>
> ------------------------------
>
> Message: 8
> Date: Mon, 23 Feb 2004 16:43:32 -0500
> From: "Elaine Dooley"
> Subject: [Histonet] Perforin
> To:
> Message-ID:
> Content-Type: text/plain; charset=US-ASCII
>
> Dear histonetters,
>
> Does anyone out there have a antibody called Perforin that works well in
> formalin fixed paraffin embedded tissues?
>
>
> Elaine Dooley
> Shands Teaching Hospital
> Gainesville FL
>
> 352-265-0111 ext 72117
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Mon, 23 Feb 2004 16:48:22 -0500
> From: Ramona Tolliver
> Subject: [Histonet] Yale University - Opening
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <6.0.1.1.2.20040223164739.01dca2b8@email.med.yale.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
> I'm resending this due to trouble with the URL for the position. It can
> also be reached by going to www.yale.edu/jobs and searching Pathology.
>
> Thanks,
>
> Ramona
>
>
> ~~~~~~~~~~~
>
> Good afternoon,
>
> Yale University has a 2nd Shift Histology Supervisor Position available=20
> from 3:00 -11:00 p.m. If you know of anyone who may be interested in a=20
> great opportunity with advancement potential, please forward this
> information on to them.
>
> The posting is located at :
> http://websrv.its.yale.edu/hr/cgi-bin/printjob.plx?file=mp+2&job=151919.
>
> Salary starts at 65K and is commensurate with experience. Relocation
> assistance is available.
>
> Thank you for your time!
>
> Ramona Tolliver
>
>
> Ramona E. Tolliver
> Human Resource Manager
> Yale University School of Medicine
> Department of Pathology
> 310 Cedar Street
> New Haven, CT 06520-8023
> Telephone: (203) 785-6689
> Fax: (203) 785-7303
>
> ------------------------------
>
> Message: 10
> Date: Mon, 23 Feb 2004 14:55:05 -0700
> From: marilyn.johnson@gov.ab.ca
> Subject: [Histonet] CMC mounting media
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; charset=us-ascii
>
> Hi Histonetters,
> I am looking for a "CMC" combined stain/mounting medium. Apparently, this
> soln. can be pretinted with acid fuchsin or aniline blue to the mounting
> medium. This soln. is required to demonstrate minute radulae.
> Any replies would be greatly appreciated.
> Thanks in advance.
>
> Marilyn Johnson
> Alberta Agriculture
> Edmonton, Alberta, Canada
>
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Tue, 24 Feb 2004 12:46:18 +1300
> From: "Jeremy Browne"
> Subject: [Histonet] unsubscribe please
> To:
> Message-ID: <006401c3fa67$3ba6d2b0$91c7d882@ssc.auckland.ac.nz>
> Content-Type: text/plain; charset="us-ascii"
>
> 4th attempt!!
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy
> Maronto
> Sent: Monday, February 23, 2004 9:21 AM
> To: histonet@lists.utsouthwestern.edu; Myri37@aol.com
> Subject: Re: [Histonet] MMA
>
> Myriam,
> I have used the MMA/butylmethacrylate/methylbenzoate/peg 400/ benzoyl
> peroxide (BPO) combination to embed undecalcified bone and small stented
> arteries. It is an easy combination to make up and use. I expect you
> have three changes suggested in your procedure. Mix each about 4 hours
> before use. It seems I have the best results with this. The
> infiltration time will have to be worked out and if vacuum is used, it
> should be limited to a couple of hours after each change of plastic. I
> make prepolymerized containers to place the tissue on and add the
> embedding plastic on. It will keep down the bubbles. When embedding,
> the air in the embedding container with the liquid plastic will need to
> be flushed out with C02 (easiest to use), you can also use gaseous
> nitrogen. It takes one to two days for complete polymerization in the
> freezer.
> Things that can cause problems with polymerization:
> Containers not flushed enough that have oxygen in them will not fully
> polymerize. I place the flushed embedding containers in a bell jar,
> flush jar with CO2 and seal. This goes in the freezer.
> Humid conditions can cause poorly polymerization ---some hard--some
> still liquid. Usually have to replace the embedding plastic, flush with
> CO2 and try again. You can use drierite in the bottom to help this.
>
> After the blocks are hard, the plastic is trimmed a little to the block
> size I want to use in the microtome. I wet cut 4 micron sections and
> place them on a chrome-gelatin coated slide with a couple of drops of
> 70% alcohol, on a slide warmer. The section flattens out, I put a piece
> of plastic on it, gently roll with a small wall paper roller. when I
> get them done I clamp them with c-clamps. It is good idea to have a
> small piece of wood and extra glass slide on each end. It prevents
> slides from breaking. They get placed in the oven overnight. The
> clamps removed, plastic removed, they are ready to deplasticize with
> xylene and ready for staining. You may want to deplasticize with other
> agents such as 2-methoxyethyl acetate for immunostaining. Your
> procedure may give you more info for immunostaining. I remember doing
> Brdu with success, however, we would get a halo effect around the
> possitive cells, and I could not find anyone that had a solution to
> this. I did find th
> at
> certain antibody diluents and blockers were a key in the success of
> this.
>
> I hope this helps.
> Nancy
> MPI Research
> Mattawan, MI
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 12
> Date: Mon, 23 Feb 2004 20:50:44 EST
> From: Linresearch@aol.com
> Subject: [Histonet] (no subject)
> To: histonet@pathology.swmed.edu
> Message-ID: <1e9.19a5a825.2d6c07f4@aol.com>
> Content-Type: text/plain; charset="US-ASCII"
>
> Hello,
> I am still trying to find a CD21 that works on FFPE rat tissues.
> I would appreciate any suggestions.
> Lin
>
>
> ------------------------------
>
> Message: 13
> Date: Mon, 23 Feb 2004 20:52:24 EST
> From: Linresearch@aol.com
> Subject: [Histonet] CD21
> To: histonet@pathology.swmed.edu
> Message-ID: <8a.4306d47.2d6c0858@aol.com>
> Content-Type: text/plain; charset="US-ASCII"
>
> Hello,
> I am still tryinmg to find a CD21 that works on FFPE rat tissues.
> Any suggestions would be appreciated.
> Lin
>
>
> ------------------------------
>
> Message: 14
> Date: Tue, 24 Feb 2004 10:16:44 +0000
> From: Shaumik Adhya
> Subject: [Histonet] cutting frozen sections of myocardium
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <1077617804.403b248cc5e70@www.webmail.ucl.ac.uk>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi Histonetters,
>
> I'm a clinician trying to get to grips with histotechnology techniques for
>
> research. I'm having a lot of difficulty cutting frozen sections of rat
> myocardium. I seem to be cutting sections with a lot of holes in them,
> and
> the fibres seem poorly arrayed. I'm cutting rat myocardium to practice
> before
> human myocardial biopsies.
>
> The rats are killed, the hearts harvested, without fixation. They're cut
> into
> small pieces before staining for 20 hours in senescence associated beta=20
> galactosidase solution (essentially x-gal solution at pH 6). They are
> then
> moutned into OCT, frozen in isopentane and cut on a cryotome at -20
> centigrade. The slides are then fixed in 3% paraformaldehyde, wawshed in
> distilled water, before Haemotxylin & Eosin staining.
>
> Any advice, tips or tricks will be much appreciated.
>
> Shaumik Adhya
>
>
>
>
>
> ------------------------------
>
> Message: 15
> Date: Tue, 24 Feb 2004 12:46:29 +0100
> From: Nancy.Walker@sanofi-synthelabo.com
> Subject: [Histonet] filtering stains (what paper?)
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; charset=iso-8859-1
>
>
> What grade of filter paper is good for filtering hemalun? The paper we
> recently bough(Whatman 113V) seems to retain too much (very slow filtering
> with rapid loss of staining quality).
>
> How many times do you refilter stains before discarding?
>
> thanks again,
>
> Nancy Walker
> Molecular Biology Scientist
>
> Sanofi-Synthelbo Research
> B.P. 37 Labége Innopole
> 31676 LABEGE CEDEX FRANCE
>
> nancy.walker@sanofi-synthelabo.com
> tel : (33)561004179 fax :(33)561004001
>
>
>
>
>
>
> ------------------------------
>
> Message: 16
> Date: Tue, 24 Feb 2004 12:51:30 -0000
> From: Mike Bromley
> Subject: [Histonet] substance p
> To: "Histonet (E-mail)"
> Message-ID: <7325D637DFE2D211928800902733A7F303B94A40@DGAMTBDCEMS>
> Content-Type: text/plain
>
> Hi All
> Has anyone immunostained for substance P on formalin fixed paraffins. I
> would appreciate info re: antibody type and source, retrieval etc
>
> Best Wishes
>
> Mike Bromley
>
> Chief Biomedical Scientist
> Pathology
> Dumfries & Galloway Royal Infirmary
> Scotland, UK
>
> > This e-mail and any files transmitted with it are private and intended
> > solely for the use of the individual or entity to whom they are
> addressed.
> > If you have received this e-mail in error please return it to the
> address
> > it
> > came from telling them it is not for you and then delete it from your
> > system.
> >
> >
>
>
> ------------------------------
>
> Message: 17
> Date: Tue, 24 Feb 2004 06:51:28 -0600
> From: "Nita Searcy"
> Subject: [Histonet] Isopentane
> To:
> Message-ID: <04Feb24.065158cst.119053@healthcare2.sw.org>
> Content-Type: text/plain; charset=US-ASCII
>
> For Vinny- or anyone. I am trying to substitute liquid nitrogen for
> Isopentane in the frozen lab due to volatility. Could not access
> Histologic article from May regarding laboratory accident. Anyone
> have any other incidents? Help would be appreciated.
>
>
>
> ------------------------------
>
> Message: 18
> Date: Tue, 24 Feb 2004 14:23:50 +0100
> From: Nancy.Walker@sanofi-synthelabo.com
> Subject: [Histonet] filtering stains (what paper?)
> To: Histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; charset=iso-8859-1
>
>
> ----- Réacheminé par Nancy Walker/FR-LABEGE/RESEARCH/SANOFI le 24/02/04
> 14:14 -----
>
>
> Nancy Walker
>
> Pour :
> histonet@lists.utsouthwestern.edu
> 24/02/04 12:46 cc :
>
> Objet : filtering stains
> (what paper?)(Document link: Nancy Walker)
>
>
>
>
>
> What grade of filter paper is good for filtering hemalun? The paper we
> recently bough(Whatman 113V) seems to retain too much (very slow filtering
> with rapid loss of staining quality).
>
> How many times do you refilter stains before discarding?
>
> thanks again,
>
> Nancy Walker
> Molecular Biology Scientist
>
> Sanofi-Synthelbo Research
> B.P. 37 Labége Innopole
> 31676 LABEGE CEDEX FRANCE
>
> nancy.walker@sanofi-synthelabo.com
> tel : (33)561004179 fax :(33)561004001
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 19
> Date: Tue, 24 Feb 2004 08:37:17 -0500
> From: Myri37@aol.com
> Subject: [Histonet] (no subject)
> To: histonet@pathology.swmed.edu
> Message-ID: <0F0551F2.71392D2B.0005167B@aol.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> hello
> i'ev heard of villanueva stain, that is a good stain for bonea
> do you have any protocol, how to prepare it ? or where could i buy it ?=20
> thank you very much
> Myriam
>
>
>
> ------------------------------
>
> Message: 20
> Date: Tue, 24 Feb 2004 08:45:00 -0500
> From: Gary Gill
> Subject: RE: [Histonet] filtering stains (what paper?)
> To: "'Nancy.Walker@sanofi-synthelabo.com'"
> ,
> Histonet@lists.utsouthwestern.edu
> Cc: histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; charset="iso-8859-1"
>
> Filtration per se does not cause "rapid loss of staining quality" --
> regardless of pore size. However, the "very slow filtering" you've
> described suggests you're using Harris hematoxylin, which typically throws
> a
> surface precipitate when exposed to atmospheric oxygen. That precipitate
> is
> actually aluminum-hematein, which is the active complex responsible for
> staining. It forms because oxygen continues to oxidize initially
> unoxidized
> hematoxylin, which forms so much more aluminum-hematein that it exceeds
> its
> solubility limit in water. Repeatedly removing this complex by filtering
> over-and-over again causes the rapid loss of staining quality.
>
> Mixing three parts of Harris hematoxylin with 1 part ethylene glycol fixes
> the problem. Aluminum-hematein is several times more soluble in 20%
> ethylene glycol than it is in water.
>
> Gary Gill
>
> -----Original Message-----
> From: Nancy.Walker@sanofi-synthelabo.com
> [mailto:Nancy.Walker@sanofi-synthelabo.com]
> Sent: Tuesday, February 24, 2004 8:24 AM
> To: Histonet@lists.utsouthwestern.edu
> Cc: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] filtering stains (what paper?)
>
>
>
> ----- Réacheminé par Nancy Walker/FR-LABEGE/RESEARCH/SANOFI le 24/02/04
> 14:14 -----
>
>
> Nancy Walker
>
> Pour :
> histonet@lists.utsouthwestern.edu
> 24/02/04 12:46 cc :
>
> Objet : filtering stains
> (what paper?)(Document link: Nancy Walker)
>
>
>
>
>
> What grade of filter paper is good for filtering hemalun? The paper we
> recently bough(Whatman 113V) seems to retain too much (very slow filtering
> with rapid loss of staining quality).
>
> How many times do you refilter stains before discarding?
>
> thanks again,
>
> Nancy Walker
> Molecular Biology Scientist
>
> Sanofi-Synthelbo Research
> B.P. 37 Labége Innopole
> 31676 LABEGE CEDEX FRANCE
>
> nancy.walker@sanofi-synthelabo.com
> tel : (33)561004179 fax :(33)561004001
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 21
> Date: Tue, 24 Feb 2004 08:45:00 -0500
> From: Gary Gill
> Subject: RE: [Histonet] filtering stains (what paper?)
> To: "'Nancy.Walker@sanofi-synthelabo.com'"
> ,
> Histonet@lists.utsouthwestern.edu
> Cc: histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; charset="iso-8859-1"
>
> Filtration per se does not cause "rapid loss of staining quality" --
> regardless of pore size. However, the "very slow filtering" you've
> described suggests you're using Harris hematoxylin, which typically throws
> a
> surface precipitate when exposed to atmospheric oxygen. That precipitate
> is
> actually aluminum-hematein, which is the active complex responsible for
> staining. It forms because oxygen continues to oxidize initially
> unoxidized
> hematoxylin, which forms so much more aluminum-hematein that it exceeds
> its
> solubility limit in water. Repeatedly removing this complex by filtering
> over-and-over again causes the rapid loss of staining quality.
>
> Mixing three parts of Harris hematoxylin with 1 part ethylene glycol fixes
> the problem. Aluminum-hematein is several times more soluble in 20%
> ethylene glycol than it is in water.
>
> Gary Gill
>
> -----Original Message-----
> From: Nancy.Walker@sanofi-synthelabo.com
> [mailto:Nancy.Walker@sanofi-synthelabo.com]
> Sent: Tuesday, February 24, 2004 8:24 AM
> To: Histonet@lists.utsouthwestern.edu
> Cc: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] filtering stains (what paper?)
>
>
>
> ----- Réacheminé par Nancy Walker/FR-LABEGE/RESEARCH/SANOFI le 24/02/04
> 14:14 -----
>
>
> Nancy Walker
>
> Pour :
> histonet@lists.utsouthwestern.edu
> 24/02/04 12:46 cc :
>
> Objet : filtering stains
> (what paper?)(Document link: Nancy Walker)
>
>
>
>
>
> What grade of filter paper is good for filtering hemalun? The paper we
> recently bough(Whatman 113V) seems to retain too much (very slow filtering
> with rapid loss of staining quality).
>
> How many times do you refilter stains before discarding?
>
> thanks again,
>
> Nancy Walker
> Molecular Biology Scientist
>
> Sanofi-Synthelbo Research
> B.P. 37 Labége Innopole
> 31676 LABEGE CEDEX FRANCE
>
> nancy.walker@sanofi-synthelabo.com
> tel : (33)561004179 fax :(33)561004001
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 22
> Date: Tue, 24 Feb 2004 10:10:18 -0600
> From: "Atoska S. Gentry"
> Subject: Fwd: [Histonet] cutting frozen sections of myocardium
> To: Histonet
> Message-ID:
> <6.0.1.1.0.20040224100656.025955a0@mailhost.vetmed.auburn.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
>
> Hello is it possible for you to fix overnight or for 24hrs and then
> cryoprotect in sucrose/fixative or sucrose/PB before freezing for
> cryosectioning? If you know that fixation won't destroy the enzyme you
> might try this method. Best wishes. Atoska
>
> >Date: Tue, 24 Feb 2004 10:16:44 +0000
> >From: Shaumik Adhya
> >To: histonet@lists.utsouthwestern.edu
> >User-Agent: Internet Messaging Program (IMP) 3.2.1
> >X-Originating-IP: 128.40.253.73
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> and
> > related fields
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> >,
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> >Subject: [Histonet] cutting frozen sections of myocardium
> >X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on
> swlx162.swmed.edu
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> >
> >Hi Histonetters,
> >
> >I'm a clinician trying to get to grips with histotechnology techniques
> for
> >research. I'm having a lot of difficulty cutting frozen sections of rat
> >myocardium. I seem to be cutting sections with a lot of holes in them,
> and
> >the fibres seem poorly arrayed. I'm cutting rat myocardium to practice
> >before
> >human myocardial biopsies.
> >
> >The rats are killed, the hearts harvested, without fixation. They're cut
>
> >into
> >small pieces before staining for 20 hours in senescence associated beta
> >galactosidase solution (essentially x-gal solution at pH 6). They are
> then
> >moutned into OCT, frozen in isopentane and cut on a cryotome at -20
> >centigrade. The slides are then fixed in 3% paraformaldehyde, wawshed in
> >distilled water, before Haemotxylin & Eosin staining.
> >
> >Any advice, tips or tricks will be much appreciated.
> >
> >Shaumik Adhya
> >
> >
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> Atoska S. Gentry B.S., HT(ASCP)
> Research Assistant III
> Scott-Ritchey Research Center
> College of Veterinary Medicine
> Auburn University, AL 36849
> Phone# (334)844-5579 Fax# (334)844-5850
>
>
>
>
> ------------------------------
>
> Message: 23
> Date: Tue, 24 Feb 2004 10:22:25 -0600 (CST)
> From: "John C. Dennis"
> Subject: Re: [Histonet] substance p
> To: Mike Bromley
> Cc: "Histonet \(E-mail\)"
> Message-ID:
> Content-Type: TEXT/PLAIN; charset=US-ASCII
>
> Mike
>
> I used a rabbit polyclonal from Chemicon. It worked well.
>
> John Carroll Dennis
> Anatomy, Physiology, and Pharmacology
> 109 Greene Hall
> Auburn University, AL 36849
>
>
> On Tue, 24 Feb 2004, Mike Bromley wrote:
>
> > Hi All
> > Has anyone immunostained for substance P on formalin fixed paraffins. I
> > would appreciate info re: antibody type and source, retrieval etc
> >
> > Best Wishes
> >
> > Mike Bromley
> >
> > Chief Biomedical Scientist
> > Pathology
> > Dumfries & Galloway Royal Infirmary
> > Scotland, UK
> >
> > > This e-mail and any files transmitted with it are private and intended
> > > solely for the use of the individual or entity to whom they are
> addressed.
> > > If you have received this e-mail in error please return it to the
> address
> > > it
> > > came from telling them it is not for you and then delete it from your
> > > system.
> > >
> > >
> > _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
>
>
> ------------------------------
>
> Message: 24
> Date: Tue, 24 Feb 2004 10:12:15 -0600
> From: "Atoska S. Gentry"
> Subject: Fwd: [Histonet] Isopentane
> To: Histonet
> Message-ID:
> <6.0.1.1.0.20040224101051.0259e070@mailhost.vetmed.auburn.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
>
> Hello, I don't believe isopentane can be substituted for liquid nitrogen,
> they're usually used in conjunction. However, dry ice can be substituted
> for liquid nitrogen. Best wishes. Atoska
>
> >X-Mailer: Novell GroupWise Internet Agent 6.5.1
> >Date: Tue, 24 Feb 2004 06:51:28 -0600
> >From: "Nita Searcy"
> >To:
> >X-Scan-Signature: 8550f81978efdbf6f5d10beda515049b
> >X-BeenThere: histonet@lists.utsouthwestern.edu
> >X-Mailman-Version: 2.1.3
> >List-Id: For the exchange of information pertaining to histotechnology
> and
> > related fields
> >List-Unsubscribe:
> >,
> >
> >
> >List-Archive:
> >List-Post:
> >List-Help:
>
> >List-Subscribe:
> ,
> >
>
> >Sender: histonet-bounces@lists.utsouthwestern.edu
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> >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu
> >Subject: [Histonet] Isopentane
> >X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on
> swlx162.swmed.edu
> >X-Spam-Level:
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> version=2.63
> >X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003)
> >X-SA-Exim-Scanned: Yes
> >
> >For Vinny- or anyone. I am trying to substitute liquid nitrogen for
> >Isopentane in the frozen lab due to volatility. Could not access
> >Histologic article from May regarding laboratory accident. Anyone
> >have any other incidents? Help would be appreciated.
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> Atoska S. Gentry B.S., HT(ASCP)
> Research Assistant III
> Scott-Ritchey Research Center
> College of Veterinary Medicine
> Auburn University, AL 36849
> Phone# (334)844-5579 Fax# (334)844-5850
>
>
>
>
> ------------------------------
>
> Message: 25
> Date: Tue, 24 Feb 2004 12:02:30 -0500
> From: "Martha Ward"
> Subject: [Histonet] please unsubscribe
> To:
> Message-ID:
>
> <61135F0455D33347B5AAE209B903A304076A4DD5@EXCHVS2.medctr.ad.wfubmc.edu>
>
> Content-Type: text/plain; charset="us-ascii"
>
> I will out of the lab from 2/24/2004 to 3/8/2004
> Martha Ward
> Wake Forest University Baptist Medical Center
>
>
> ------------------------------
>
> Message: 26
> Date: Tue, 24 Feb 2004 11:23:43 -0600
> From: "Atoska S. Gentry"
> Subject: Fwd: Re: Fwd: [Histonet] Isopentane
> To: Histonet
> Message-ID:
> <6.0.1.1.0.20040224112043.025921c0@mailhost.vetmed.auburn.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
>
> Hello, I'm not aware of a procedure for quick freezing in isopentane
> alone,
> we quick freeze by immerging the sample in isopentane chilled by liquid=20
> nitrogen. Please will you share with me your protocol for quick freezing
> in
> isopentane. Thanks, Atoska
>
>
> >X-Mailer: Novell GroupWise Internet Agent 6.5.1
> >Date: Tue, 24 Feb 2004 10:50:58 -0600
> >From: "Nita Searcy"
> >To:
> >Subject: Re: Fwd: [Histonet] Isopentane
> >
> >Isopentane is used to "quick freeze" frozen sections- can't liquid
> >nitrogen be use in its place? No specialty work (muscles, etc.) but
> >routine frozens.
> >
> > >>> "Atoska S. Gentry" 02/24/04 10:12AM
> > >>>
> >
> >Hello, I don't believe isopentane can be substituted for liquid
> >nitrogen,
> >they're usually used in conjunction. However, dry ice can be
> >substituted
> >for liquid nitrogen. Best wishes. Atoska
> >
> > >X-Mailer: Novell GroupWise Internet Agent 6.5.1
> > >Date: Tue, 24 Feb 2004 06:51:28 -0600
> > >From: "Nita Searcy"
> > >To:
> > >X-Scan-Signature: 8550f81978efdbf6f5d10beda515049b
> > >X-BeenThere: histonet@lists.utsouthwestern.edu
> > >X-Mailman-Version: 2.1.3
> > >List-Id: For the exchange of information pertaining to histotechnology
> >and
> > > related fields
> > >List-Unsubscribe:
> > >,
> > >
> > >
> > >List-Archive:
> > >List-Post:
> > >List-Help:
> >
> > >List-Subscribe:
> >,
> > >
> >
> > >Sender: histonet-bounces@lists.utsouthwestern.edu
> > >X-Scan-Signature: 2f26e54c73378134ed276f0a1e9fcee8
> > >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu
> > >Subject: [Histonet] Isopentane
> > >X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on
> >swlx162.swmed.edu
> > >X-Spam-Level:
> > >X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no
> >version=2.63
> > >X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003)
> > >X-SA-Exim-Scanned: Yes
> > >
> > >For Vinny- or anyone. I am trying to substitute liquid nitrogen for
> > >Isopentane in the frozen lab due to volatility. Could not access
> > >Histologic article from May regarding laboratory accident. Anyone
> > >have any other incidents? Help would be appreciated.
> > >
> > >_______________________________________________
> > >Histonet mailing list
> > >Histonet@lists.utsouthwestern.edu
> > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >Atoska S. Gentry B.S., HT(ASCP)
> >Research Assistant III
> >Scott-Ritchey Research Center
> >College of Veterinary Medicine
> >Auburn University, AL 36849
> >Phone# (334)844-5579 Fax# (334)844-5850
> >
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> Atoska S. Gentry B.S., HT(ASCP)
> Research Assistant III
> Scott-Ritchey Research Center
> College of Veterinary Medicine
> Auburn University, AL 36849
> Phone# (334)844-5579 Fax# (334)844-5850
>
>
>
>
> ------------------------------
>
> Message: 27
> Date: Tue, 24 Feb 2004 11:40:54 -0600
> From: "Barry R Rittman"
> Subject: RE: Re: Fwd: [Histonet] Isopentane
> To:
> Message-ID:
> <566FB0B522443D43AF02D2ADBE35A6F077FCA4@UTHEVS3.mail.uthouston.edu>
> Content-Type: text/plain; charset="us-ascii"
>
>
> The purpose of using isopentane cooled with liquid nitrogen is to
> dissipate heat quickly and thus prevent the formation of a vapor barrier
> around the tissues. When nitrogen is used alone, the vapor barrier that
> is formed significantly decreases the rate of cooling and therefore
> increases the possibility of ice crystal formation and tissue damage.
> Barry
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Atoska
> S. Gentry
> Sent: Tuesday, February 24, 2004 11:24 AM
> To: Histonet
> Subject: Fwd: Re: Fwd: [Histonet] Isopentane
>
>
>
> Hello, I'm not aware of a procedure for quick freezing in isopentane
> alone,
> we quick freeze by immerging the sample in isopentane chilled by liquid=20
> nitrogen. Please will you share with me your protocol for quick freezing
> in
> isopentane. Thanks, Atoska
>
>
> >X-Mailer: Novell GroupWise Internet Agent 6.5.1
> >Date: Tue, 24 Feb 2004 10:50:58 -0600
> >From: "Nita Searcy"
> >To:
> >Subject: Re: Fwd: [Histonet] Isopentane
> >
> >Isopentane is used to "quick freeze" frozen sections- can't liquid
> >nitrogen be use in its place? No specialty work (muscles, etc.) but
> >routine frozens.
> >
> > >>> "Atoska S. Gentry" 02/24/04 10:12AM
> > >>>
> >
> >Hello, I don't believe isopentane can be substituted for liquid
> >nitrogen, they're usually used in conjunction. However, dry ice can be
> >substituted
> >for liquid nitrogen. Best wishes. Atoska
> >
> > >X-Mailer: Novell GroupWise Internet Agent 6.5.1
> > >Date: Tue, 24 Feb 2004 06:51:28 -0600
> > >From: "Nita Searcy"
> > >To:
> > >X-Scan-Signature: 8550f81978efdbf6f5d10beda515049b
> > >X-BeenThere: histonet@lists.utsouthwestern.edu
> > >X-Mailman-Version: 2.1.3
> > >List-Id: For the exchange of information pertaining to
> > >histotechnology
> >and
> > > related fields
> > >List-Unsubscribe:
> > >,
> > >
> > > > >>
> > >List-Archive:
> > >List-Post:
> > >List-Help:
> >
> > >List-Subscribe:
> >,
> > >
> >
> > >Sender: histonet-bounces@lists.utsouthwestern.edu
> > >X-Scan-Signature: 2f26e54c73378134ed276f0a1e9fcee8
> > >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu
> > >Subject: [Histonet] Isopentane
> > >X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on
> >swlx162.swmed.edu
> > >X-Spam-Level:
> > >X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no
> >version=2.63
> > >X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003)
> > >X-SA-Exim-Scanned: Yes
> > >
> > >For Vinny- or anyone. I am trying to substitute liquid nitrogen for
> > >Isopentane in the frozen lab due to volatility. Could not access
> > >Histologic article from May regarding laboratory accident. Anyone
> > >have any other incidents? Help would be appreciated.
> > >
> > >_______________________________________________
> > >Histonet mailing list
> > >Histonet@lists.utsouthwestern.edu
> > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >Atoska S. Gentry B.S., HT(ASCP)
> >Research Assistant III
> >Scott-Ritchey Research Center
> >College of Veterinary Medicine
> >Auburn University, AL 36849
> >Phone# (334)844-5579 Fax# (334)844-5850
> >
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> Atoska S. Gentry B.S., HT(ASCP)
> Research Assistant III
> Scott-Ritchey Research Center
> College of Veterinary Medicine
> Auburn University, AL 36849
> Phone# (334)844-5579 Fax# (334)844-5850
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 28
> Date: Tue, 24 Feb 2004 12:52:45 -0500
> From: Dana Settembre
> Subject: Re: [Histonet] substance p
> To: M.Bromley@dgri.scot.nhs.uk, histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; charset=US-ASCII
>
> Hello Mike,
> I have stained for Substance P using a company I had never used before;
> The Arnel Products Co.
> P. O. Box 20045/ New York, NY 10021 800-333-6078
>
> I used it with DakoCytomation's ready to use Prot. K for 5 min. at room
> temp.
> The dilution I used was at 1:3000, using a human brain as a control.
>
> Next time I might try Chemicon, as the other person on the Histonet
> mentioned.
>
> Good Luck
> Dana Settembre
> University Hospital - UMDNJ
> Newark, NJ
>
>
> >>> Mike Bromley 2/24/2004 4:51:30 AM >>>
> Hi All
> Has anyone immunostained for substance P on formalin fixed paraffins.
> I
> would appreciate info re: antibody type and source, retrieval etc
>
> Best Wishes
>
> Mike Bromley
>
> Chief Biomedical Scientist
> Pathology
> Dumfries & Galloway Royal Infirmary
> Scotland, UK
>
> > This e-mail and any files transmitted with it are private and
> intended
> > solely for the use of the individual or entity to whom they are
> addressed.
> > If you have received this e-mail in error please return it to the
> address
> > it
> > came from telling them it is not for you and then delete it from
> your
> > system.
> >
> >
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> _______________________________________________
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>
> End of Histonet Digest, Vol 3, Issue 30
> ***************************************
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