[Histonet] In-situ hybridization for HPV

From:"Barnhart, Tammy"

I have been asked to develop in-situ testing for HPV on cervical biopsies.
Our cytology lab already performs HPV testing on PAP smears.  We have not,
in the past, performed this or had our cervical biposies sent out to other
labs for this testing.  So, I'm curious as to how may labs perform this
testing.  I am also wondering, for labs who do this testing, do you perform
in-situ HPV screening and then in-situ sub-typing testing or only a
screening test?  The procedures I have reviewed do not seem to be very
standardized.  I have seen procedures that mimic our immunohistochemical
bench closely and some that are quite different.  If you perform this
testing, I would be interested in your protocols.  Any and all input would
be very much appreciated.

Tammy Barnhart, BS, HTL(ASCP)
Anatomic Pathology Supervisor
St. Alexius Medical Center
Bismarck, ND

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