All:
 
 I have some dry ice frozen human brain samples that I would like to
section for IHC. We do not have a Cryostat in our lab, but we do have a
freezing microtome. Can the frozen brain chunks be fixed in 4% PF,
cryoprotected in 30% sucrose and then sectioned on the microtome for
free floating IHC? I have never cut tissue that has been fixed after
freezing, only tissue that has been fixed immediately after removal. 
 
Thanks,
 
Joe
 
-----------------------------
Joseph Burdo, Ph.D.
Postdoctoral Associate
Cellular Neurobiology Laboratory
The Salk Institute for Biological Studies
10010 N. Torrey Pines Rd.
La Jolla, CA, 92037
 
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