We do not dehydrate and clear any IFA stained sections, they are mounted
with a suitable aqueous mounting media with some using Molecular Probes
mounting medias (there are two) that are superb for many fluorophores.  

At 03:08 AM 2/6/2004 +0000, you wrote:
>Hi everyone,
>
>Does anyone have any experience with using fluorescent-labelled 
>antibodies with a fluorophore that is NOT soluble in clearing agent 
>benzyl alcohol/benzyl benzoate (BABB)?  I have had success with Texas 
>Red , but have found the following soluble: FITC, GFP, Alexa 488, Cy2.
>
>Any tips??
>
>Thanks very much.
>April
>
>-- 
>April DeLaurier, PhD
>Division of Developmental Biology
>National Institute for Medical Research
>The Ridgeway, Mill Hill
>London NW7 1AA
>United Kingdom
>Tel: +44 208 959 3666 ext. 2095
>Fax: +44 208 816 4477
>E-mail: adelaur@nimr.mrc.ac.uk
>
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>
>
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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