Re: [Histonet] Fite's Acid-Fast Stain for Nocardia
We do the Fite's routinely with a little modification.
After xylene/peanut oil, we put the slides in xylene for 10
minutes, then wash in running water. NO BLOTTING. The stain
comes out as clean as the Kinyoun's procedure for both
Nocardia and Leprosy with no loss of staining.
> We use the fite's stain for nocardia. We are having
> trouble with blotchy background staining due to the
> xylene/peanut oil used in the stain. We blot our slides
> and rinse them with warm water, but still have the
> background blotchy staining. Thank-you for any help that
> you could give me.
> Iowa State
> College of
> Veterinary Medicine
> Iowa 50011
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