RE: [Histonet] mouse perfusion

From:"Favara, Cynthia (NIH/NIAID)"


I use a butterfly needle to perfuse, roll up some 4x4 gauze to tiny
cylinders. Support the body of the mouse with the rolls of gauze and tape
the needle with white surgical tape. You can perfuse to your hearts
content-tee hee!

Cynthia Favara
903 South 4th Street
Hamilton, MT 59840

-----Original Message-----
From: Cooperman, Sharon (NIH/NICHD) 
Sent: Thursday, February 12, 2004 5:12 PM
To: ''
Subject: [Histonet] mouse perfusion

Dear Histoneteers,

I am having trouble with mouse perfusion.  We do an intracardiac (left
ventricle perfusion).  I start out fine, but about halfway through the
perfusion I get a bad hand cramp (I have writer's cramp) and can't hold the
needle well.  I often wind up punching through the septum or the anterior
wall and the rest of the perfusion doesn't go well, so I get good flushing
of blood but poor fixation.  Does anyone know of a method for holding the
needle without hand fatigue?  There are people in my lab who don't have
writer's cramp who have much less trouble with perfusions, but I can't
change the writer's cramp stuff.


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