At my former lab we tried it with Helicobacter with no success.  We did
have success with another attempt.  I think it was with AFB, but it may
have been GMS.  My memory is a little hazy this morning as I haven't had
my coffee yet.

Ross M Stapf
Histopathology Manager
Baylor University Medical Center
3500 Gaston Ave.
Dallas, TX 75246
214-820-2465

214-820-4110 fax
RossS@baylorhealth.edu



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evelyn
Kaplan
Sent: Monday, February 09, 2004 9:49 PM
To: Therersa Stegall
Cc: Histonet
Subject: RE: [Histonet] control tissues


Hi Theresa,

Thanks for your reply but I am well familiar with the 'in-house' sytem
of control tissues. I am more interested in inocultaing tissues, such as
fresh placenta, with 'substances' to create a new control block. Just
wondering if anyone has tried it!

Regards,
Evelyn

-----Original Message-----
From: Therersa Stegall [mailto:STEGTM@samcstl.org]
Sent: Tuesday, February 10, 2004 12:00 AM
To: ekaplan@squ.edu.om
Subject: Re: [Histonet] control tissues


Evelyn:  We make many of our own controls, using other cases that are
positive for the pathology tested for.  Our pathologists keep us
informed on + Helicobacter Pylori, and for other things, we use tissue
raided from surgicals (such as appendix for Trichrome, non-neoplastic
spleen for reticulum, liver for PAS).  Good liver for Fe controls is
hard to find, some Histonetters in England sent me a batch.  I think you
could "raid" surgicals, and find nearly any tissue you need for
controls.  Our computer system allows a natural language search, so it
gets easier.  Peace,  Terre

>>> "Evelyn Kaplan"  2/8/2004 12:13:37 AM >>>


Good morning,

I realise this is a subject which has been previously discussed but I
wonder if anyone has had experience of 'manufacturing' their own control
blocks? I have a student about to start a project  and would apreciate
any protocols.

Regards,
Evelyn Kaplan,
Dept of Pathology,
College of Medicine and Health Sciences,
Sultan Qaboos University,
Oman


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