RE: [Histonet] Image Analysis Software

From:"Elizabeth Chlipala"


If he has a mac available he could use NIH image, which is free.  But if
you have image pro plus that would be ideal to use.  He could load the
software on his pc and then he would need the key, which would be on the
back of the computer that has the image pro plus on it.  He could get
another key, since you already have a license for image pro plus, but I
believe that costs around $2000.00, I would check with your image pro
vendor.  If there is no issue with the transfer of the key, then that
might be the way to go.  I have worked with both NIH image and image pro
plus.  NIH image works ok if you are dealing with only one color, I have
found that you can use the grey scale image for this.  I have not been
able to work with multiple color images very well in NIH image.  An
example of this is counting nuclei that have been stained with
Hematoxylin and then counting lets say Brdu positive cells that are DAB
stained, I could not figure out a way to do this with NIH image (maybe
someone with more experience could), but I was able to do this with
image pro plus.  

Whatever program he chooses to use, it is going to take some time to
figure out how it will all work.  I hope this helps some and good luck.


Elizabeth A. Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592 
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
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University of Colorado
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-----Original Message-----
[] On Behalf Of Pravda,
Sent: Monday, February 02, 2004 10:57 AM
Subject: [Histonet] Image Analysis Software

We have a post-doc in our lab that is looking for a Windows-based
software for image analysis, specifically pixel analysis, of his
confocal images. He is doing colocalization studies of his
fluorescent-labeled tissue and is looking for a program that is less
than $500 that he can load on his personal computer. We have Image-Pro
Plus in the lab, but I am not familiar with other programs (let alone
this one) and was wondering if anyone has any suggestions for us.
Thanks so much!!
Elke Pravda
Research Assistant
Biostructure Core Facility
Forsyth Institute
Boston, MA 02155

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