RE: [Histonet] IHC on H&E section

From:"DiCarlo, Margaret"

Patsy,

To make the coverslip come off faster, fill a coplin jar with xylene and put
the slides in, cover with a top and then put the coplin jar into a 60 degree
C. oven and agitate the xylene occaisionally and the coverslip will come off
in much less time than soaking at room temperature. It's a little trick I've
learned.

Peggy DiCarlo HT(ASCP)

-----Original Message-----
From: Patsy Ruegg [mailto:pruegg@colobio.com]
Sent: Wednesday, February 11, 2004 10:36
To: Histonet@Pathology. Swmed. Edu
Cc: Ihcrg@Yahoogroups. Com
Subject: [Histonet] IHC on H&E section


I know this has come up before but don't remember the details.  I would like
to do IHC for AE1/AE3 on a slide from a study set that has been H&E stained
and coverslipped for some time now.  Please advise.  Is there a preferred
method for removing the coverslip and maintaining the section integrety?
Destaining the H&E?  Doing the IHC stain on this section?
Patsy


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