Next time I get a perm I'll have my hair dresser put me in the fume hood...j-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of BillSinaiSent: Monday, February 16, 2004 5:34 PMTo: histonet (E-mail)Subject: RE: [Histonet] GMS StainPerming solution contains thyoglycolate, if you purchase this in pure formyou then dilute it to about 1% and use it the same way as perming solution.Just remember to dilute it in the fume cupboard as the smell is atrocious.Bill SinaiLaboratory ManagerTissue Pathology, ICPMRWestmead NSW 2145AustraliaPh 02 9845 7774-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems,JoyceSent: Tuesday, 17 February 2004 9:10 AMTo: Histonet@lists.utsouthwestern.eduSubject: RE: [Histonet] GMS StainI've had a few questions regarding the perm solution. I knew I had readsomething somewhere - p445 Sheehan! She says to remove silver stains fromyour hands with perm solution, so I thought it should work on tissue aswell. It has been quite a while ago. I believe I used it full strengh andkept water close by to swish and dip until it looked just right. j:>)-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bonner,JanetSent: Tuesday, February 10, 2004 11:16 AMTo: 'Joyce Cline'; Histonet@lists.utsouthwestern.eduSubject: RE: [Histonet] GMS Stain     To remove excess silver ,we dip the slides in 4% Chromic acid and rinsein 1% sodium bisulfite, then check under the scope. If too light, start atthe silver step again.   Remember that generally a solution is first in theprocess because it affects the remaining steps, either to enhance or remove!                                   Janet-----Original Message-----From: Joyce Cline [mailto:jcline@wchsys.org]Sent: Thursday, February 05, 2004 3:39 PMTo: Histonet@lists.utsouthwestern.eduSubject: Fw: [Histonet] GMS StainI use a coplin jar of distilled water and I add two -three drops of cloroxand then dip the slides until the excess silver comes off. Rinse well inwater.----- Original Message -----From: "Poteete, Jacquie A." To: Sent: Thursday, February 05, 2004 1:58 PMSubject: [Histonet] GMS Stain> I know there is something to use when the slides get left in the silver> solution to long but have forgotten. Can someone refresh my memory?> Thanks>>>>> ********* Email Confidentiality Statement *********> Visit http://www.saintfrancis.com/emailconf.asp>> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet>>_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonetThe information contained in this message may be privileged and/orconfidential and protected from disclosure.  If the reader of this messageis not the intended recipient or agent responsible for delivering thismessage to the intended recipient, you are hereby notified that anydissemination, distribution or copying of this communication is strictlyprohibited.  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