RE: [Histonet] GMS Stain

From:"Weems, Joyce"

I've had a few questions regarding the perm solution. I knew I had readsomething somewhere - p445 Sheehan! She says to remove silver stains fromyour hands with perm solution, so I thought it should work on tissue aswell. It has been quite a while ago. I believe I used it full strengh andkept water close by to swish and dip until it looked just right. j:>)-----Original Message-----From:[]On Behalf Of Bonner,JanetSent: Tuesday, February 10, 2004 11:16 AMTo: 'Joyce Cline'; Histonet@lists.utsouthwestern.eduSubject: RE: [Histonet] GMS Stain     To remove excess silver ,we dip the slides in 4% Chromic acid and rinsein 1% sodium bisulfite, then check under the scope. If too light, start atthe silver step again.   Remember that generally a solution is first in theprocess because it affects the remaining steps, either to enhance or remove!                                   Janet-----Original Message-----From: Joyce Cline []Sent: Thursday, February 05, 2004 3:39 PMTo: Histonet@lists.utsouthwestern.eduSubject: Fw: [Histonet] GMS StainI use a coplin jar of distilled water and I add two -three drops of cloroxand then dip the slides until the excess silver comes off. Rinse well inwater.----- Original Message ----- From: "Poteete, Jacquie A." To: Sent: Thursday, February 05, 2004 1:58 PMSubject: [Histonet] GMS Stain> I know there is something to use when the slides get left in the silver> solution to long but have forgotten. Can someone refresh my memory?> Thanks>>>>> ********* Email Confidentiality Statement *********> Visit>> _______________________________________________> Histonet mailing list>>>>_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.edu information contained in this message may be privileged and/orconfidential and protected from disclosure.  If the reader of this messageis not the intended recipient or agent responsible for delivering thismessage to the intended recipient, you are hereby notified that anydissemination, distribution or copying of this communication is strictlyprohibited.  If you have received this communication in error, please notifythe sender immediately by replying to this message and deleting the materialfrom any computer. _______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.edu Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer.Thank you. Saint Josephs Health System, Inc.

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