RE: [Histonet] Cutting 20-50 micron sections
Depending on your tissue, you may wish to consider embedding in a lower melting point paraffin wax. This will generally enable you to cut much thicker sections will less curling and cracking.
You can lower the MP of paraffin wax with a variety of additives such as beeswax or buy a lower MP wax from the supplier.
Other approaches would be to use a thin layer beeswax on the top and bottom of the block for better adherence between sections as they are being cut so that they remain flatter; or use a block of lower MP wax wiping a thin layer of this on the surface of the block between sections. The latter is very time consuming and you have to be careful to be squeaky clean but it helps especially with sections that tend to crack.
From: firstname.lastname@example.org on behalf of Featherstone, Annette
Sent: Fri 2/13/2004 5:14 AM
To: Histonet (E-mail)
Subject: [Histonet] Cutting 20-50 micron sections
Any suggestions for cutting thick (20-50) micron paraffin sections. They
curl when they hit the blade and though I am perservering, I was hoping for
a tip or two, or three or four or five .................
This email transmission and any documents, files,
or previous e-mail messages attached to it are
confidential and intended solely for the use of the
individual or entity to whom they are addressed.
If you are not the intended recipient, or a person
responsible for delivering it to the intended recipient,
you are hereby notified that any further review,
disclosure, copying, dissemination, distribution, or
use of any of the information contained in or attached
to this e-mail transmission is strictly prohibited.
If you have received this message in error, please
notify the sender immediately by e-mail, discard
any paper copies, and delete all electronic files
of the message. If you are unable to contact the
sender or you are not sure as to whether you
are the intended recipient, please e-mail
ISTSEC@KaleidaHealth.org or call (716) 859-7777.
Histonet mailing list
<< Previous Message | Next Message >>