[Histonet] museum specimens
I have a question regarding the preparation of museum specimens for histology.
I recently obtained access to several museum specimens (formalin fixed and
then preserved in 70% ethanol) that we are using for a comparative
neuroanatomical study. We are hoping to successfully extract the brains and
section the tissue to examine cerebellar structure. I was thinking of taking
the brains through a graded series of ethanols to PBS, post-fixing in PFA and
gelatin embedding them for frozen sections. Alternatives would be to leave it
in 70% ethanol and section it on a vibratome or paraffin embed them.
Does anyone have any experience in performing histology on museum specimens
like these or suggestions/advice on the matter?
Andrew N. Iwaniuk
Post-doctoral Research Fellow
Songbird Neuroethology Lab
Department of Psychology
University of Alberta
P217 Biological Sciences Building
Edmonton, Alberta, T6G 2E9
Ph: +1 780 492 0323
Fax: +1 780 492 1768
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