[Histonet] immunoflourescent positive signal around tissue edges only

From:"Brent Benish"

Having an issue where my liver tissue only has positive immunofluorescent
labeling near the lateral edges of the tissue.  As I look medially, the
positive signal is lost.
This effect is dramatic in paraffin embedded tissues, and less dramatic with
frozen sections that are postfixed on slides with 4% para.  Sections are
covered with solutions for all protocol steps, and are not drying out.  Any
ideas about why only the outer edges (5 cells deep) of the liver label
properly???

Thanks
Brent

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