[Histonet] freezing microtome disposable blade holder
This is my first experience using histonet. It was suggested to me that I
might find through histonet, a blade holder for our very old (but still
working well)American Optical 860 freezing microtome. We are presently
using a permanant steel blade that needs sharpening periodically. We would
like to try using disposable blades but have no disposable blade holder for
Here are my two questions.
1)Does anyone know where we might purchase a blade holder that will fit our
microtome? It is the grandfather of microtomes.
2)Would we be compromising quality of the sectioned slice by using
disposable blades instead of using our permanent blade?
We use the microtome for slicing frozen paraformaldehyde-fixed rodent brain
tissue at 30 to 80 um thickness.
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