[Histonet] aniline blue for trichrome

From:"Elizabeth Chlipala"

Hello all
We are having a problem with our trichrome stain, we are not getting the
correct differentiation of muscle.  We normally don't have any problems
with this stain, since we always use fresh reagents.  We had an old
bottle of aniline blue from EMS that we have been using, I had ordered a
new bottle from Harleco they are both the same CI - 42755.  We started
having problems with the new aniline blue, but I'm not certain that this
is the reason why since what is not staining in the tissues is the
smooth muscle of vessels.  We have run this stain on rat and mouse
livers, both from carbon tetrachloride induced liver toxicity studies,
the morphological changes in this model include fibrosis, necrosis and
degenerating hepatocytes.  The rat livers look fine, except that the
smooth muscle of the vessels are not staining red.  The mouse livers
also have this problem, but in addition to that the degenerating
hepatocytes are staining slightly blue.  Also, when I checked into
ordering additional aniline blue from Sigma they use Methyl Blue, water
soluable - certified for use as Aniline Blue, but the CI is different
its 42780.  Any suggestions would be helpful.  
Thanks in advance
Elizabeth A. Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592 
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
Premier Histology Laboratory
University of Colorado
MCBD, Room A3B40
Boulder, Colorado 80309
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