[Histonet] Zymed monoclonal Ab to B-galactosidase (ZBG1 clone)

From:"PALMER Jason (SVHM)"


	Hi Histonetters.
	I'm trying to get the above antibody working with immunohistochemistry, but without any luck. I have tried FFPE tissues, with different pretreatment / retrieval steps, and snap frozen tissue, cut then fixed in acetone or paraformaldehyde, all to no avail. I'm using the DAKO ARK kit since I'm using mouse antibody on mouse (transgenic) tissue. Has anybody had success of any sort with this antibody? Any responses much appreciated.
	Jason Palmer
	Bernard O'Brien Institute of Microsurgery 
	42 Fitzroy St, Fitzroy Victoria 3065 
	tel +61 3 9288 4018 
	fax +61 3 9416 0926 
	email: palmerj@svhm.org.au 

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