[Histonet] Why not just make TRIS buffer in the lab

From:Gayle Callis

Instead of making PBS, make up TRIS buffered saline.  Make up a 10X
solution of TRIS, dilute when you need it 1:10, extremely easy. 

Here is Chris van der Loos recipe that is excellent, simple - just test pH
of 10X - we dilute it 1:10, and the pH is always right on the nose. 

10X TRIS (0.5M) Buffered Saline (8.9% to 9%)

TRIS base				60.57g/liter
Sodium chloride			89 - 90g
Conc Hydrochloric acid		30 ml
Test pH approx 7.8

We store stock 10X in refrigerator, others may not. 

Working 0.05M TBS

Dilute Stock 10X 1:10 with pure water.

We buy our TRIS base from Fisher, with a huge discount.  So the buffer is
cost effective.  


Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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