[Histonet] Why not just make TRIS buffer in the lab
Instead of making PBS, make up TRIS buffered saline. Make up a 10X
solution of TRIS, dilute when you need it 1:10, extremely easy.
Here is Chris van der Loos recipe that is excellent, simple - just test pH
of 10X - we dilute it 1:10, and the pH is always right on the nose.
10X TRIS (0.5M) Buffered Saline (8.9% to 9%)
TRIS base 60.57g/liter
Sodium chloride 89 - 90g
Conc Hydrochloric acid 30 ml
Test pH approx 7.8
We store stock 10X in refrigerator, others may not.
Working 0.05M TBS
Dilute Stock 10X 1:10 with pure water.
We buy our TRIS base from Fisher, with a huge discount. So the buffer is
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
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