Vinnie, 

I see your point, I didn't indicate volume of water the salts are dissolved
in! It is rewritten! 

TRIS base is Tris(hydorymethl)aminomethane, F.W. aka MW 121.14. 

61 g TRIS base and 90 g NaCl is added to approx 800 ml water (these salts
go into water very easily!  You could use a smaller volume, I have seen 600
ml also), then add 30 mls HCL and bring to final volume (qs) to 1 liter
with distilled water. Check pH just before final volume to leave room for
either more HCl or sodium hydroxide for pH adjustment.

 

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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