[Histonet] Revco

From:Dana Settembre

A Revco is a brand of deep freezers ,  usually around -80 degrees celcius.
Dana Settembre
University Hospital, UMNDJ
Newark, NJ

>>> "Marshall Terry Dr, Consultant Histopathologist"  2/16/2004 8:49:21 AM >>>
A Revco being?

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire
 England
        terry.marshall@rothgen.nhs.uk 

-----Original Message-----
From: Bill Blank [mailto:bill501@mindspring.com] 
Sent: 16 February 2004 16:34
To: Jennifer Hoover; histonet@lists.utsouthwestern.edu 
Subject: Re: [Histonet] Storage of Primary Antibodies!


At 8:48 AM -0500 2/16/04, Jennifer Hoover wrote:
>  I have a question regarding the storage (short or longer
>term) of primary antibodies.

IN the good days before monoclonals and kits we 
made GFAP the old fashion wat by injecting 
rabits, harvesting antibodies and absorbing until 
we got good specific staining. About a litre of 
good antibody was aliquoted and stored in a Revco 
(I think -70) and it remained good for at least 
8 years.

If I had a Revco now, I would do this will all 
antibodies and validate them per case by using 
controls.
-- 
_______________
Bill Blank, MD
Heartland Lab, Inc

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