[Histonet] Re: mouse perfusion
I do perfusions on rats, and I place a surgical clamp over the heart to fix the needle (blunt, 18 gauge) in place. The clamp I use resembles this: http://www.edmo.com/s14/42H.asp , but has a curved nose. The needle is attached to a tube supplying the solutions; this keeps the liquid reservoirs well out of the way. I use an electrical peristaltic pump to push the liquids through.
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