[Histonet] Re: help with criostat HM 505 E (mauricio velandia)
Hi,
We had that problem when someone from another lab turned off our
cryostat. There should be a blank button on the control panel probably
under the lightbulb button. You just need to press that and it should
restart it. The cryostat needs to cool down that's why the error
message occurs.
hope this helps
Sandy Thevarkunnel
Boston University School of Medicine
On Monday, February 2, 2004, at 01:00 PM, histonet-
request@lists.utsouthwestern.edu wrote:
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> Today's Topics:
>
> 1. Justification for Tape Coverslipper (Cheasty, Sandra)
> 2. help with criostat HM 505 E (mauricio velandia)
> 3. help with criostat HM 505 E (mauricio velandia)
> 4. RE: combined ISH/IHC (Mikael Niku)
> 5. Re: IHC validation (John Auld)
> 6. BCl2 on Ventana Benchmark (Fearn Tony)
> 7. Wanted dead or alive - tissue negative for connexin 43
> (Louise Carrington)
> 8. RE: BCl2 on Ventana Benchmark (GUTIERREZ, JUAN)
> 9. TMA Software. (LD Ridley)
> 10. TMA Software. (LD Ridley)
> 11. Re: TMA Software. (Helen Fedor)
> 12. RE: Justification for Tape Coverslipper (Browning Deb)
> 13. RE: TMA Software. (Luis Chiriboga)
> 14. Fwd: [Histonet] pregnancy in histology lab (Atoska S. Gentry)
> 15. RE: pregnancy in histology lab (Bonner, Janet)
> 16. Antibody search (Hermina Borgerink)
> 17. Re: pregnancy in lab (Jackie.O'Connor@abbott.com)
> 18. Re: Re: pregnancy in lab (Andrea Grantham)
> 19. Slide labeling pens (Richard Cartun)
> 20. RE: Re: "Need your help" (Horn, Hazel V)
> 21. RE: Slide labeling pens (Gary Gill)
> 22. RE: Slide labeling pens (Michelle Becker)
> 23. Image Analysis Software (Pravda, Elke)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sun, 1 Feb 2004 13:46:40 -0800
> From: "Cheasty, Sandra"
> Subject: [Histonet] Justification for Tape Coverslipper
> To: "HistoNet \(E-mail\)"
> Message-ID: <2E50F33F91EEDA46A77BC3B2575BB091058757@mars.llumc.edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
> The staff here can hand coverslip 30 slides in 8-10 minutes
> The Sakura tape coverslipper does 30 in 1.5 minutes.
> With our workload this is about 2 hours a day in tech time that would
> be saved.
> When this tech time cost savings is weighed against the cost of the
> machine and the increase in consumables (tape vs. coverslips and
> mounting medium) the savings does not seem that overwhelming, even over
> 5 years.
>
> Am I missing some factor in this equation? I truly love the Sakura
> coverslipper, have used it in two other labs, and even wrote a
> justification for one, but that was in 1992. Has the cost of the tape
> gone up that much? I remember a much bigger savings. Is my memory
> failing? Any input you experts can give me would be much
> appreciated! (except those who blame my failing memory...)
>
> Thanks
>
> Confidentiality Note:
>
> The preceding e-mail message (including any attachments) contains
> information that may be confidential, protected by applicable legal
> privileges, or constitute non-public information. It is intended to be
> conveyed only to the designated recipient(s). If you are not an
> intended recipient of this message, please notify the sender by
> replying to this message and then delete it from your system. Use,
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> unintended recipients is not authorized and may be unlawful.
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Sun, 01 Feb 2004 23:15:52 -0500
> From: "mauricio velandia"
> Subject: [Histonet] help with criostat HM 505 E
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; charset=iso-8859-1; format=flowed
>
> Hello to every body, excuse me for my english, it`s no very good.
>
> I need your help, I have a criostat Microm HM 505 E, but I haven`t
> service
> or user manual and when I turn on the equipment appear error E-03,and I
> don¥t know what the meaning this error code, if is possible and
> somebody can
> help me say me what is this error?, Thanks you.
>
> Maurice Velandia
> technics
> Bogot· D.C. - Colombia
>
> _________________________________________________________________
> Consigue aquÌ las mejores y mas recientes ofertas de trabajo en AmÈrica
> Latina y USA: http://latam.msn.com/empleos/
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Sun, 01 Feb 2004 23:21:16 -0500
> From: "mauricio velandia"
> Subject: [Histonet] help with criostat HM 505 E
> To: Histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; charset=iso-8859-1; format=flowed
>
> Excuse me I don¥t say what is my e-mail to answer about error Code E-03
> in
> Hm 505 E of Microm criostat.
>
> My e-mail is maovel70@hotmail.com
>
> Thanks for your answers.
>
> _________________________________________________________________
> MSN Amor: busca tu Ω naranja http://latam.msn.com/amor/
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Mon, 2 Feb 2004 09:47:00 +0200
> From: "Mikael Niku"
> Subject: RE: [Histonet] combined ISH/IHC
> To: "'Tora Bardal'" ,
>
> Message-ID: <000101c3e960$c0dcbd90$8c0fd680@ekk1116>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Dear Tora,
>
> we are routinely doing combined ISH + IHC. The ISH is for genomic DNA,
> so with a RNA target this might be slightly more tricky. But RNA in
> tissues is surprisingly well preserved, so I think this should work.
>
> I spent a LOT of time trying to find a useful protocol for a double
> staining. The only generally useful way I know is to use the tyramide
> signal amplification system.
>
> This is how it goes:
>
> 1) Start with IHC: antigen retrieval, primary antibody, biotinylated /
> HRP-conjugated secondary antibody, and then the tyramide reaction. Now
> you have a covalently bound label (biotinylated tyramide, for example)
> in the tissue, so you don't need to worry about antigen destruction or
> stripping of bound antibodies.
>
> 2) Then perform the whole ISH procedure.
>
> 3) After you have completed the NBT/BCIP color reaction of ISH, finish
> the IHC (if using biotinylated tyramide, add HRP-avidin, then DAB).
>
> This protocol allows you to begin with IHC (to avoid destruction of
> antigens in the harsh ISH treatments) but to do the visualization step
> only after ISH (to avoid false negatives caused by the DAB precipitate).
> As additional bonus, you get signal amplification for IHC, and a nicely
> even DAB color intensity (nice provided that you don't need any idea of
> quantitation).
>
> If it's the RNA that is the more sensitive target, I guess you could do
> this the other way round (to use tyramide for RNA detection).
>
> The only drawbacks I can think of are:
>
> 1) A few additional incubations due to the tyramide protocol (these are
> quick to do, though)
> 2) The commercial tyramide reagents (which you need to use at least if
> you're doing any commercial stuff, as the technology is patented) are
> pretty expensive, if you're doing lots of slides.
>
> Please let me know if you would like to get a copy of our exact
> protocol.
>
> +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
> + Mikael Niku
> + University of Helsinki, Dept. Basic Veterinary Sciences
> + URL: www.helsinki.fi/~mniku/
> +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
>
> - Mit‰kˆ mielt‰ olen l‰nsimaisesta sivistyksest‰?
> Minusta se olisi erinomainen ajatus!
> (Gandhi)
>
>
>
>> -----Original Message-----
>> From: histonet-bounces@lists.utsouthwestern.edu
>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf
>> Of Tora Bardal
>> Sent: 28. tammikuuta 2004 17:16
>> To: histonet@lists.utsouthwestern.edu
>> Subject: [Histonet] combined ISH/IHC
>>
>>
>> Hello
>> Does anyone have a working protocol they can share?
>>
>> I'm trying to combine in situ hybridization (RNAprobe) and
>> immunohistochemistry (PCNA) on fish paraffin sections.
>> So far: ISH ok, IHC doesn't work, or ISH no signal, IHC ok.
>> PCNA(DAKO)
>> needs AR.
>>
>> Before spending more time an money I would like to have some
>> good advices
>> on what to do when, ab simultaneous incubation? stepwise?
>> crossreactions?
>> blocking ?
>> I'm using sheep anti-DIG-AP/BCIP/NBT (ISH) and DAKO Envision
>> HRP (mouse)/DAB.
>>
>> Tora
>>
>>
>> Tora Bardal
>> Department of Biology,
>> Norwegian University of Science and Technology (NTNU)
>> Bratt¯ra Research Center
>> N-7491 Trondheim
>> Norway + (47)73 59 09 38 / 970 25256
>> E-mail: Tora.Bardal@bio.ntnu.no fax:+ (47)73 59 63 11
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listin> fo/histonet
>>
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 2 Feb 2004 09:35:03 +0000
> From: "John Auld"
> Subject: [Histonet] Re: IHC validation
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; charset=us-ascii
>
> Joyce
>
> In the UK there is a National EQA scheme, which also has non UK based
> labs
> enrolled, for IHC. There are several schemes run by the body, eg
> general,
> lymphoma, cytology, breast etc. EQA participation is a requirement for
> accreditation in the UK and if the inspectors think we should be in
> more or
> different schemes will register this as a non compliance. The EQA body
> also
> run meetings and workshops.
>
> John
>
> Date: Fri, 30 Jan 2004 14:18:18 -0500
> From: "Joyce Cline"
> Subject: [Histonet] Validation for IHC
> To:
> Message-ID: <002001c3e765$d180d3e0$1d2a14ac@wchsys.org>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Does anyone send their positive control tissue to another lab for
> validation of their immuno staining?
> The path that is over the immuno area wants me to varify the use
> of my
> patient controls by sending to another lab to show that these controls
> are
> positive by another source.
> Has anyone been CAP inspected and asked if they do this? Seems he
> thinks an inspector is going to ask this.
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Mon, 2 Feb 2004 11:37:47 -0000
> From: Fearn Tony
> Subject: [Histonet] BCl2 on Ventana Benchmark
> To: "Histonet (E-mail)"
> Message-ID: <8C3DE9069A06D611B8950002A550C15943BD4E@BHC_MAIL02>
> Content-Type: text/plain; charset="ISO-8859-1"
>
> We've been trying to optimise our antibodies on our Ventana Benchmark.
> At
> the moment we've been trying to use the DAKO antibody but it's not been
> that
> fantastic. Does anyone use the Ventana one, and is it any good?
> Lesley
>
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Mon, 02 Feb 2004 12:57:25 +0000
> From: "Louise Carrington"
> Subject: [Histonet] Wanted dead or alive - tissue negative for
> connexin 43
> To:
> Message-ID:
> Content-Type: text/plain; charset=US-ASCII
>
> hello ,
> I am trying to find a tissue negative for connexin 43 (Cx43) to
> preadsorb an
> antibody with and/or use as a negative staining tissue - i have been
> using it to
> immuno-stain fresh-frozen and frozen/fixed (ethanol, acetone, 1%, 4%
> pfa) and
> have ended up with nuclear staining in rat, human and bovine tissue. it
> looks
> 'real' ie heterogenous cell-cell staining, its not my secondary - as
> ommission
> of primary yeilds no background (i'm using alexafluor fluorescent
> secondaries)
> or my fixation as all fixation prtocols including no-fix show the same
> and i've
> found it in several differnet organs (whilst searching for a positive
> control)
> in several different species...... but the antibody is to a
> phosphorylated
> isoform of cx43 and my my pan connexin 43 antibody shows no nuclear
> staining at
> all. The phospho-cx43 has not been published yet and the company have
> been
> ignoring my emails so if anyone can suggest a tissue (or even cell
> culture as i
> get staining here as well) that has nucleate Connexin43 cells within it
> that are
> either
>
> a) abundant and easy to isolate so that i can preadsorb the antibody OR
>
> b) easy to recognise so that i can do immunostaining
>
> (or god help me ... both??)
> i would be really grateful,
>
> i have been searching but cx 43 is a tad ubiquitous :)
>
> thankyou in advance
>
> louise
>
>
>
> Louise Carrington PhD.
> Cell & Molecular Unit,
> Department of Optometry & Vision Sciences,
> Cardiff University,
> Redwood Building,
> King Edward VII Ave.,
> Cathays Park,
> Cardiff,
> Wales,
> UK
>
> Tel 02920 875665
> email: CarringtonL@cardiff.ac.uk
>
>
>
> ------------------------------
>
> Message: 8
> Date: Mon, 2 Feb 2004 08:12:18 -0600
> From: "GUTIERREZ, JUAN"
> Subject: RE: [Histonet] BCl2 on Ventana Benchmark
> To: "Fearn Tony" , "Histonet
> (E-mail)"
> Message-ID:
>
> Content-Type: text/plain; charset="utf-8"
>
> Yes we do, and yes it is. We use CC1 standard, 28 min. primary and A/B
> block. Comes out beatiful. Good luck,
>
> Juan
>
> -----Original Message-----
> From: Fearn Tony [mailto:Tony.Fearn@cd.burnleyhc-tr.nwest.nhs.uk]
> Sent: Mon 2/2/2004 5:37 AM
> To: Histonet (E-mail)
> Cc:
> Subject: [Histonet] BCl2 on Ventana Benchmark
>
>
>
> We've been trying to optimise our antibodies on our Ventana
> Benchmark. At
> the moment we've been trying to use the DAKO antibody but it's not
> been that
> fantastic. Does anyone use the Ventana one, and is it any good?
> Lesley
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 9
> Date: Mon, 02 Feb 2004 14:20:37 +0000
> From: LD Ridley
> Subject: [Histonet] TMA Software.
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <1075731637.af1d4400L.D.Ridley@bham.ac.uk>
> Content-Type: text/plain; charset="UTF-8"
>
> This is just an enquiry to all those out there that construct and
> analyse Tissue Microarrays in the lab. We are looking into the various
> analysis software packages availible to try and organise our data we
> are rapidly generating. I would be really grateful if people could let
> me know what they are using, where it can be obtained from including
> costs etc and any other opinions you have of it.
> Many thanks in advance for your help
> Lee
> Lee Ridley
> Research Associate
>
> L.D.Ridley@bham.ac.uk
> Cancer Research UK Institute for Cancer Studies
> University of Birmingham
> Vincent Drive
> Edgbaston
> Birmingham
> B15 2TT
> UK
>
> +44 (0)121 414 4472
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Mon, 02 Feb 2004 14:25:50 +0000
> From: LD Ridley
> Subject: [Histonet] TMA Software.
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <1075731950.af1d4400L.D.Ridley@bham.ac.uk>
> Content-Type: text/plain; charset="UTF-8"
>
> This is just an enquiry to all those out there that construct and
> analyse Tissue Microarrays in the lab. We are looking into the various
> analysis software packages availible to try and organise our data we
> are rapidly generating. I would be really grateful if people could let
> me know what they are using, where it can be obtained from including
> costs etc and any other opinions you have of it.
> Many thanks in advance for your help
> Lee
> Lee Ridley
> Research Associate
>
> L.D.Ridley@bham.ac.uk
> Cancer Research UK Institute for Cancer Studies
> University of Birmingham
> Vincent Drive
> Edgbaston
> Birmingham
> B15 2TT
> UK
>
> +44 (0)121 414 4472
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Mon, 02 Feb 2004 09:45:17 -0500
> From: Helen Fedor
> Subject: Re: [Histonet] TMA Software.
> To: L.D.Ridley@bham.ac.uk, histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; charset=US-ASCII
>
> http://webhost5.nts.jhu.edu/~tmalab/
> this is our website.
> We have our own software, Which was designed and written by our lab.
> It is pretty user friendly. The data for the array design is put into
> the array builder application. After the slides are stained they can be
> scanned with an image analysis system, and the images are then directly
> linked to the data from the scan.
> you can get to our software from this page.
> you can view a demo of the software and also log on as guest to view
> images.
> Please let me know if you need any further assistance.
>
> Helen
>
>
> Helen L. Fedor B.S.
> Johns Hopkins University
> Brady Urological Institute
> 600 N Wolfe St
> Marburg Room 406
>
> WARNING: E-mail sent over the Internet is not secure. Information sent
> by e-mail may not remain confidential.
> DISCLAIMER: This e-mail is intended only for the individual to whom it
> is addressed. It may be used only in accordance with applicable laws. If
> you received this e-mail by mistake, notify the sender and destroy the
> e-mail.
>
> Baltimore MD 21287
> email: hfedor@jhmi.edu
> Phone: 410 614-1660
> Pager: 410 283-3419
>
>
>>>> LD Ridley 02/02/04 09:25AM >>>
> This is just an enquiry to all those out there that construct and
> analyse Tissue Microarrays in the lab. We are looking into the various
> analysis software packages availible to try and organise our data we are
> rapidly generating. I would be really grateful if people could let me
> know what they are using, where it can be obtained from including costs
> etc and any other opinions you have of it.
> Many thanks in advance for your help
> Lee
> Lee Ridley
> Research Associate
>
> L.D.Ridley@bham.ac.uk
> Cancer Research UK Institute for Cancer Studies
> University of Birmingham
> Vincent Drive
> Edgbaston
> Birmingham
> B15 2TT
> UK
>
> +44 (0)121 414 4472
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 12
> Date: Mon, 2 Feb 2004 09:48:32 -0500
> From: Browning Deb
> Subject: RE: [Histonet] Justification for Tape Coverslipper
> To: "'Cheasty, Sandra'" , "HistoNet (E-mail)"
>
> Message-ID:
> <3AADFB88753AD31189C100902786B91C0E277FE6@hch_nt_exchange.hhsc.ca>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Our main concern, while we do find the tape expensive, is the fact that
> the
> tape coverslips "remove" themselves after a few years, often removing
> the
> entire section with it, leaving us with drawers full of blank slides and
> loose coverslip/tissue combo's. At this point we need to recut the
> original
> block. The beauty of this system is that we can ship our slides within
> minutes of coverslipping.
>
> -----Original Message-----
> From: Cheasty, Sandra [mailto:SCheasty@ahs.llumc.edu]
> Sent: Sunday, February 01, 2004 4:47 PM
> To: HistoNet (E-mail)
> Subject: [Histonet] Justification for Tape Coverslipper
>
>
> The staff here can hand coverslip 30 slides in 8-10 minutes
> The Sakura tape coverslipper does 30 in 1.5 minutes.
> With our workload this is about 2 hours a day in tech time that would be
> saved.
> When this tech time cost savings is weighed against the cost of the
> machine
> and the increase in consumables (tape vs. coverslips and mounting
> medium)
> the savings does not seem that overwhelming, even over 5 years.
>
> Am I missing some factor in this equation? I truly love the Sakura
> coverslipper, have used it in two other labs, and even wrote a
> justification
> for one, but that was in 1992. Has the cost of the tape gone up that
> much?
> I remember a much bigger savings. Is my memory failing? Any input you
> experts can give me would be much appreciated! (except those who
> blame my
> failing memory...)
>
> Thanks
>
> Confidentiality Note:
>
> The preceding e-mail message (including any attachments) contains
> information that may be confidential, protected by applicable legal
> privileges, or constitute non-public information. It is intended to be
> conveyed only to the designated recipient(s). If you are not an intended
> recipient of this message, please notify the sender by replying to this
> message and then delete it from your system. Use, dissemination,
> distribution or reproduction of this message by unintended recipients
> is not
> authorized and may be unlawful.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> This information is directed in confidence solely to the person named
> above
> and may not otherwise be distributed, copied or disclosed. Therefore,
> this
> information should be considered strictly confidential. If you have
> received this email in error, please notify the sender immediately via a
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>
>
>
>
>
> ------------------------------
>
> Message: 13
> Date: Mon, 02 Feb 2004 10:06:01 -0500
> From: Luis Chiriboga
> Subject: RE: [Histonet] TMA Software.
> To: LD Ridley ,
> histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; charset=utf-8
>
> Chih Long Lui etal American journal of Pathology V161(5)Nov02 1557-1565
> Just started using & so far not to bad. software is based on gene
> microarray analysis so it's pretty complimentary. can get more info
> from their website. It's freeware.
> http://genome-www.stanford.edu/TMA/index.shtml
>
> Luis
>
>
> --------------------------------------
> Luis Chiriboga Ph.D., HT (ASCP) QIHC
> New York University School Of Medicine
> NYU Cancer Institute and
> Bellevue Hospital Center
> Department Of Pathology 4W27
> 27th Street & First Avenue
> New York, N.Y. 10016
> (212) 562-4667.
>
>
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of LD Ridley
> Sent: Monday, February 02, 2004 9:21 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] TMA Software.
>
>
> This is just an enquiry to all those out there that construct and
> analyse Tissue Microarrays in the lab. We are looking into the various
> analysis software packages availible to try and organise our data we
> are rapidly generating. I would be really grateful if people could let
> me know what they are using, where it can be obtained from including
> costs etc and any other opinions you have of it.
> Many thanks in advance for your help
> Lee
> Lee Ridley
> Research Associate
>
> L.D.Ridley@bham.ac.uk
> Cancer Research UK Institute for Cancer Studies
> University of Birmingham
> Vincent Drive
> Edgbaston
> Birmingham
> B15 2TT
> UK
>
> +44 (0)121 414 4472
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 14
> Date: Mon, 02 Feb 2004 08:50:59 -0600
> From: "Atoska S. Gentry"
> Subject: Fwd: [Histonet] pregnancy in histology lab
> To: Histonet
> Message-ID:
> <6.0.1.1.0.20040202084903.025ba2a0@mailhost.vetmed.auburn.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
>
>> Date: Fri, 30 Jan 2004 12:38:05 -0800 (PST)
>> From: Stacey Burton
>> To: histonet@pathology.swmed.edu
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>> Subject: [Histonet] pregnancy in histology lab
>> X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on
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>>
>> I am performing a pole for some inquiring employees. If anyone would
>> like
>> to comment - Thank you.
>>
>> 1. Have you ever been pregnant while working with in the histology
>> laboratory? Yes, twice.
>>
>> 2. Did your work duties have to be altered while being pregnant and to
>> what extent? No.
>>
>> 3. Did working in the histology laboratory effect your pregnancy in
>> any
>> negative way? No.
>
>>
>> Our laboratory does perform regular ppm testing for xylene and
>> formalin.
>>
>> Thank you for any comments for our pole,
>>
>> Stacey L. Burton, H.T. ASCP
>> Laboratory Manager
>> Unipath LLC
>> San Antonio TX
>> 210) 521-7700
>> staceylburton@yahoo.com
>>
>>
>>
>>
>>
>> ---------------------------------
>> Do you Yahoo!?
>> Yahoo! SiteBuilder - Free web site building tool. Try it!
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> Atoska S. Gentry B.S., HT(ASCP)
> Research Assistant III
> Scott-Ritchey Research Center
> College of Veterinary Medicine
> Auburn University, AL 36849
> Phone# (334)844-5579 Fax# (334)844-5850
>
>
>
>
> ------------------------------
>
> Message: 15
> Date: Mon, 2 Feb 2004 10:28:39 -0500
> From: "Bonner, Janet"
> Subject: RE: [Histonet] pregnancy in histology lab
> To: "'Gudrun Lang'" , Histonetliste
> , Stacey Burton
>
> Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB3F4B@fh2k093.fhmis.net>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I think that if anything develops later on in your children, you'll
> always
> wonder what role Histology may have played. I worked in a lab in
> 1980-81
> when I was pregnant and my son seemed fine - until last week. An Echo
> cardiogram showed a missing tricuspid valve and the other two were
> fused!
> Of course now he's 22 and been through things like strept throat and ear
> aches and God only knows what other flus, genetic abnormalities never
> discovered before ECHOs, etc. Enjoy life while we have it together,
> but
> ALWAYS take precautions no matter what you are doing-
> (My older collegues suffered as a result of choloroform which used to
> be
> widely used in Histology but is now restricted/prohibited.)
>
> -----Original Message-----
> From: Gudrun Lang [mailto:gudrun.lang@aon.at]
> Sent: Friday, January 30, 2004 5:27 PM
> To: Histonetliste; Stacey Burton
> Subject: Re: [Histonet] pregnancy in histology lab
>
>
> My children are 8 and 11 years old, and until now they are obviosly
> healthy.
> During pregnancy I was not allowed to be in rooms with formalin, xylol
> or
> any other probably cancerogen reagens. I was not allowed to handle with
> nativ probes, like making cryo sections or grossing.
> My duties were cutting in a seperate room and doing administrative jobs.
> I had a coworker, who had her children in the 1960s. In this time, there
> were no fumehoods and xylol-wet slides were dried near the radiator.
> Using
> gloves was not really appriciated. But yet her girls are healthy and
> have
> also healthy children.
> But something irritated me, when she told about the good old times. Her
> elder colleages didn't reach a really high age and often had some cancer
> disease?!
> hope this helps
>
> Gudrun Lang
> Linz, Austria
> ----- Original Message -----
> From: "Stacey Burton"
> To:
> Sent: Friday, January 30, 2004 9:38 PM
> Subject: [Histonet] pregnancy in histology lab
>
>
>> I am performing a pole for some inquiring employees. If anyone would
>> like
> to comment - Thank you.
>>
>> 1. Have you ever been pregnant while working with in the histology
> laboratory?
>>
>> 2. Did your work duties have to be altered while being pregnant and to
> what extent?
>>
>> 3. Did working in the histology laboratory effect your pregnancy in
>> any
> negative way?
>>
>> Our laboratory does perform regular ppm testing for xylene and
>> formalin.
>>
>> Thank you for any comments for our pole,
>>
>> Stacey L. Burton, H.T. ASCP
>> Laboratory Manager
>> Unipath LLC
>> San Antonio TX
>> 210) 521-7700
>> staceylburton@yahoo.com
>>
>>
>>
>>
>>
>> ---------------------------------
>> Do you Yahoo!?
>> Yahoo! SiteBuilder - Free web site building tool. Try it!
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> The information contained in this message may be privileged and/or
> confidential and protected from disclosure. If the reader of this
> message
> is not the intended recipient or agent responsible for delivering this
> message to the intended recipient, you are hereby notified that any
> dissemination, distribution or copying of this communication is strictly
> prohibited. If you have received this communication in error, please
> notify
> the sender immediately by replying to this message and deleting the
> material
> from any computer.
>
>
>
> ------------------------------
>
> Message: 16
> Date: Mon, 2 Feb 2004 10:29:29 -0500
> From: "Hermina Borgerink"
> Subject: [Histonet] Antibody search
> To: "histonet"
> Message-ID:
> <9AEEF1FB6254224AA355ED285F84916503F8EA13@EXCHVS2.medctr.ad.wfubmc.edu>
>
> Content-Type: text/plain; charset="US-ASCII"
>
> Hi all,
> One of our research investigators is looking for two antibodies: (1)
> Steroid sulfatase and (2) 17 beta hydroxysteroid dehydrogenase. I did
> an online search but came up with nothing. He was also interested in
> knowing if any of you have successfully used Cytochrome P450 Aromatase
> from Serotec on FFPE tissues. I would greatly appreciate any input you
> might be able to give me.
> Hermina
>
> Hermina M. Borgerink, BA, HTL(ASCP)QIHC
> Wake Forest University Health Sciences
> Department of Pathology
> Medical Center Blvd.
> Winston-Salem, NC 27157
> Tel. (336) 716-1538
> Fax (336) 716-1515
> e-mail hborgeri@wfubmc.edu
>
>
>
>
> ------------------------------
>
> Message: 17
> Date: Mon, 2 Feb 2004 10:08:36 -0600
> From: Jackie.O'Connor@abbott.com
> Subject: [Histonet] Re: pregnancy in lab
> To: histonet@lists.utsouthwestern.edu,
> histonet-bounces@lists.utsouthwestern.edu
> Message-ID:
> ON86256E2E.0056AC80@northamerica.intra.abbott.com>
>
> Content-Type: text/plain; charset="us-ascii"
>
> I've been working in histology since 1970. I didn't work in a lab
> during
> my first two pregnancies - my oldest daughter (25) and second daughter
> (24) were biology and art majors, respectively. I worked during my 3rd
> pregnancy (1982) in a tox research lab with no safety measures except
> for
> an exhaust hood for formalin trimming and xylene coverslipping. A
> couple
> of the tox PhD's suggested I stay away from xylene during my pregnancy,
> so
> someone else coverslipped for me - I still did the staining. That
> daughter was diagnosed with learning disabilities when she was 8 years
> old, and she continues to have problems at age 21. While working full
> time in a lab where we only had laminar flow ventilation to draw away
> xylene and formalin fumes, my fourth pregnancy ended in a fetal demise
> at
> 20 weeks. I worked full time during my next pregnancy, (1984-1985).
> That
> daughter has multiple minor congenital defects which include a mitral
> valve prolapse, scoliosis, a concave sternum, a small area of left
> temporal lobe atrophy, and a mandible deformity. She also is plagued
> with
> learning disabilities. Two subsequent pregnancies (1987, 1989) both
> resulted in second trimester fetal demise. My high risk OB in Chicago
> suggested at the time (1989) that lab chemicals may be suspect in my
> case,
> and when it ended he said "You're not going to try this again, are you?"
> As I've heard other people state, you just never know. I'll never know.
> There are other chemicals in the lab to consider as well as formalin and
> xylene, tho - aniline dyes, silver nitrate, mercury, uranyl nitrate,
> chloroform, toluene, to name a few. Personally, I would recommend any
> pregnant woman stay out of the histology lab. I wish the NSH would
> do a
> study on pregnancy in the histology lab, retrospective or otherwise.
>
> Jacqueline M. O'Connor HT(ASCP)
> Abbott Laboratories
> Global Pharmaceutical Research and Development
> Discovery Chemotheraputics
> 847.938.4919
>
>
>
> ------------------------------
>
> Message: 18
> Date: Mon, 02 Feb 2004 09:46:45 -0700
> From: Andrea Grantham
> Subject: Re: [Histonet] Re: pregnancy in lab
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> <4.3.2.7.2.20040202092832.00cafee0@algranth.inbox.email.arizona.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
> My children are 29 and 26. I wasn't working in Histology when I was
> pregnant the first time. This child has a degree in Molecular Biology
> and
> is working in a cord blood registry lab, however despite an
> exceptionally
> high IQ he was diagnosed with several learning disabilities while in
> elementary and jr. high and suffered from allergies to just about
> everything. I know it is very controversial now but ritalin was our
> salvation along with a very busy schedule. The medication didn't make
> him a
> zombie and it was an incredible help to him in the academic setting.
> Thankfully he now does better with the allergies and he does well in
> work
> situations. My second child is an archeologist with a masters degree and
> suffered only from being an "average" child. I was working in histology
> when I was pregnant with her. I asked the pathologists and lab manager
> during that time about the hazards of working around the chemicals and
> they
> told me not to be concerned - their only concern was how long the
> maternity
> leave was going to be! I took what precautions I could - it was a lot
> different back then. She was born a month early but suffered no serious
> effects.
> Andi Grantham
> .....................................................................
> : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
> : Sr. Research Specialist University of Arizona :
> : (office: AHSC 4212) P.O. Box 245044 :
> : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
> : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) :
> :...................................................................:
> http://www.cba.arizona.edu/histology-lab.html
>
>
>
>
> ------------------------------
>
> Message: 19
> Date: Mon, 02 Feb 2004 12:13:16 -0500
> From: "Richard Cartun"
> Subject: [Histonet] Slide labeling pens
> To:
> Message-ID:
> Content-Type: text/plain; charset=US-ASCII
>
> I agree that the "RediSharp PLUS!" pen is fantastic for labeling glass
> slides. Unfortunately, our supplier no long carries them. I was
> pleased to see that "Ted Pella, Inc." carries them. However, I spoke
> with them this morning and they told me that they are currently NOT
> available. Does anyone know a supplier for these pens? Thanks!
>
> Richard Cartun
>
>
>
> ------------------------------
>
> Message: 20
> Date: Mon, 2 Feb 2004 11:27:08 -0600
> From: "Horn, Hazel V"
> Subject: RE: [Histonet] Re: "Need your help"
> To: "'MTitford@aol.com'" ,
> Histonet@lists.utsouthwestern.edu
> Message-ID:
>
> Content-Type: text/plain
>
> Our hospital has a surgical case review committee as well. We don't
> to
> the reqs for no specimens though.
>
> Hazel Horn, HT/HTL (ASCP)
> Histology Supervisor
> Arkansas Children's Hospital
>
> Phone - 501.364.4240
> Fax - 501.364.3912
>
> -----Original Message-----
> From: MTitford@aol.com [mailto:MTitford@aol.com]
> Sent: Friday, January 30, 2004 4:29 PM
> To: Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Re: "Need your help"
>
>
> Nita Searcy asks about investigating possible unneeded surgical
> procedures.
> Our hospital, as part of its JCAHO accreditation (Joint Council for the
> Accretation of Hospitals Organization or something close to that)has a
> "Surgical Case Review Committee". It meets monthly and reviews suspect
> or
> unnecessary surgery's among other things. It also takes on little
> projects
> like reviewing appendix operations to make sure they are needed. Also as
> part of our accretation processes, we require a surgical pathology slip
> be
> issued for each surgery that takes place, even if there is no specimen.
> The
> idea is the pathologist reviews the slip and if he/she thinks it is
> unnecessary,or there should have been a specimen, the slip is sent on
> to the
> "Surgical Case Review Committee". The scrub nurse signs these off as
> "none"
> where the specimen should be listed. (I served on the committe for a
> couple
> of years. It was quite interesting. We sat in plush chairs in the
> hospital
> boardroom and got fed lunch....)
>
> Mike Titford
> USA Pathology
> Mobile AL USA
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> The information contained in this message may be privileged and
> confidential and protected from disclosure. If the reader of this
> message is not the intended recipient, or an employee or agent
> responsible for delivering this message to the intended recipient, you
> are hereby notified that any dissemination, distribution or copying of
> this communication is strictly prohibited. If you have received this
> communication in error, please notify us immediately by replying to the
> message and deleting it from your computer. Thank you.
>
>
>
>
> ------------------------------
>
> Message: 21
> Date: Mon, 2 Feb 2004 12:28:03 -0500
> From: Gary Gill
> Subject: RE: [Histonet] Slide labeling pens
> To: 'Richard Cartun' ,
> histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain
>
> Try Google, 86 hits for redisharp plus.
>
> -----Original Message-----
> From: Richard Cartun [mailto:Rcartun@harthosp.org]
> Sent: Monday, February 02, 2004 12:13 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Slide labeling pens
>
>
> I agree that the "RediSharp PLUS!" pen is fantastic for labeling glass
> slides. Unfortunately, our supplier no long carries them. I was
> pleased to
> see that "Ted Pella, Inc." carries them. However, I spoke with them
> this
> morning and they told me that they are currently NOT available. Does
> anyone
> know a supplier for these pens? Thanks!
>
> Richard Cartun
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 22
> Date: Mon, 2 Feb 2004 12:43:30 -0500
> From: "Michelle Becker"
> Subject: RE: [Histonet] Slide labeling pens
> To: "Richard Cartun" ,
>
> Message-ID:
> Content-Type: text/plain; charset="us-ascii"
>
> Try Staples #498428 for fine black ink - $13.08 per dozen.
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard
> Cartun
> Sent: Monday, February 02, 2004 12:13 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Slide labeling pens
>
>
> I agree that the "RediSharp PLUS!" pen is fantastic for labeling glass
> slides. Unfortunately, our supplier no long carries them. I was
> pleased to see that "Ted Pella, Inc." carries them. However, I spoke
> with them this morning and they told me that they are currently NOT
> available. Does anyone know a supplier for these pens? Thanks!
>
> Richard Cartun
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 23
> Date: Mon, 2 Feb 2004 12:56:53 -0500
> From: "Pravda, Elke"
> Subject: [Histonet] Image Analysis Software
> To:
> Message-ID:
> <25E0A02D668CF34ABA185EA5E5ABF06F01E8CC3A@exchange.forsyth.org>
> Content-Type: text/plain; charset="utf-8"
>
> We have a post-doc in our lab that is looking for a Windows-based
> software for image analysis, specifically pixel analysis, of his
> confocal images. He is doing colocalization studies of his
> fluorescent-labeled tissue and is looking for a program that is less
> than $500 that he can load on his personal computer. We have Image-Pro
> Plus in the lab, but I am not familiar with other programs (let alone
> this one) and was wondering if anyone has any suggestions for us.
>
> Thanks so much!!
>
> Elke Pravda
> Research Assistant
> Biostructure Core Facility
> Forsyth Institute
> Boston, MA 02155
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 3, Issue 2
> **************************************
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